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ABE543 Anti-PRDM16 Antibody

Anti-PRDM16, Cat. No. ABE543, is a rabbit polyclonal antibody that detects PR domain zinc finger protein 16 (PRDM16) and is teste for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-PRDM16 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: PRDM16, MEL1, PFM13 UniProt Number: Q9HAZ2
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ABE543
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P), IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE543
      DescriptionAnti-PRDM16 Antibody
      Alternate Names
      • PR domain zinc finger protein 16
      • PR domain-containing protein 16
      • Transcription factor MEL1
      • MDS1/EVI1-like gene 1
      Background InformationHistone-lysine N-methyltransferase PRDM16 (UniProt: Q9HAZ2; also known as EC:2.1.1.367, PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1) is encoded by the PRDM16 (also known as KIAA 1675, MEL1, PFM13) gene (Gene ID: 63976) in human. PDRM16 is a transcription factor with 10 CeH2-type zinc finger domains. It is highly expressed in adipocytes where it is involved in browning and thermogenesis of adipocytes, the beigeing of adipocytes, the adipogenic differentiation of myoblasts, and the conversion of visceral adipocytes to subcutaneous adipocytes. Its expression is also observed in uterus, kidney, cardiomyocytes, and interstitial cells. It is reported to control a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. Loss of PRDM16 from brown fat precursors is reported to cause a loss of brown fat characteristics and promote muscle differentiation. Conversely, ectopic expression of PRDM16 in myoblasts is shown to induce their differentiation into brown fat cells. It stimulates brown adipogenesis by binding to PPAR- and activating its transcriptional function. PDRM16 also displays histone methyltransferase activity and monomethylates lysine 9 of histone H3 (H3K9me1) in the cytoplasm. It is also known to function as a repressor of TGF-β signaling. Four isoforms of PDRM16 have been described that are produced by alternative promoter usage and alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Takahata, M., et al. (2009). J. Biol. Chem. 284(5); 3334-3344; Seale, P., et al. (2008). Nature. 454(7207); 961-967).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-PRDM16, Cat. No. ABE543, is a rabbit polyclonal antibody that detects PR domain zinc finger protein 16 (PRDM16) and is teste for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:200 dilution from a representative lot detected PRDM16 in Human kidney tissue sections.

      Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated PRDM16 in the nuclear extract of LNCap cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Histone-lysine N-methyltransferase PRDM16.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Histone-lysine N-methyltransferase PRDM16.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Horse, Guinea Pig, Canine, Bovine, Non-Human Primate based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRDM16
      • MEL1
      • PFM13
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular WeightTarget molecular weight ~140 kDa observed; 140.25 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in nuclear extract of LNCap cells.

      Western Blotting Analysis: A 1:1000 dilution of this antibody detected PRDM16 in nuclear extract of LNCap cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE543 04053252937491

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies