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07-2068 Anti-PRDM2 Antibody (RIZ1)

Use Anti-PRDM2 (RIZ1) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ELISA to detect PRDM2 (RIZ1) also known as GATA-3 binding protein G3B or Lysine N-methyltransferase 8.

Anti-PRDM2 Antibody (RIZ1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Oncoproteins Gene Symbol: RIZ , RIZ1 , RIZ2, EC 2.1.1.43, HUMHOXY1, KMT8 , MTB-ZF, OTTHUMP00000009642, OTTHUMP00000009687 UniProt Number: Q13029
07-2068
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, ELISARbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2068
      DescriptionAnti-PRDM2 Antibody (RIZ1)
      Alternate Names
      • GATA-3 binding protein G3B
      • GATA-3-binding protein G3B
      • Lysine N-methyltransferase 8
      Background InformationPRDM2, also known as RIZ1, is a nuclear protein containing 8 zinc finger motifs. It contains an RB-binding motif that is related to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C- terminus of E1A. It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined. RIZ1 is a tumor suppressor gene, and RIZ1 inactivation is commonly found in many types of human cancers. RIZ1 inactivation most frequently occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, or missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal in buffer containing 0.09% NaN3.
      Applications
      ApplicationUse Anti-PRDM2 (RIZ1) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ELISA to detect PRDM2 (RIZ1) also known as GATA-3 binding protein G3B or Lysine N-methyltransferase 8.
      Key Applications
      • Immunohistochemistry
      • ELISA
      Application NotesELISA:
      This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
      Biological Information
      ImmunogenThe immunogen was a KLH-conjugated synthetic peptide corresponding the C-terminal region of human PRDM2.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes PRDM2.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Not tested in other species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RIZ
      • RIZ1
      • RIZ2
      • EC 2.1.1.43
      • HUMHOXY1
      • KMT8
      • MTB-ZF
      • OTTHUMP00000009642
      • OTTHUMP00000009687
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.

      CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.

      SUBUNIT STRUCTURE:Binds to the retinoblastoma protein (RB). Interacts with GATA3.

      SUBCELLULAR LOCATION: Nucleus. Ref.6 Ref.1

      TISSUE SPECIFICITY: Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.

      SEQUENCE SIMILARITY: Contains 8 C2H2-type zinc fingers.

      Contains 1 SET domain.
      Molecular Weight~189 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunohistochemistry:
      1:100 dilution of this antibody detected PRDM2 in formalin-fixed, paraffin-embedded human malignant melanoma tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2068 04053252364112