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MABF3036-100UG Anti-PSGL-1/CD162 Antibody, clone HECA-452

Anti-PSGL-1/CD162, clone HECA-452, Cat. No. MABF3036, is a rat monoclonal antibody that detects P-selectin glycoprotein ligand 1 (PSGL-1) and is tested for use in Flow Cytometry, Immunohistochemistry, and Western Blotting.

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MABF3036-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3036-100UG
      DescriptionAnti-PSGL-1/CD162 Antibody, clone HECA-452
      Alternate Names
      • P-selectin glycoprotein ligand 1
      • PSGL-1 Selectin P ligand
      • ;CD162
      • P-selectin glycoprotein ligand 1
      Background InformationP-selectin glycoprotein ligand 1 (UniProt: Q14242; also known as PSGL-1 Selectin P ligand, CD162) is encoded by the SELPLG gene (Gene ID: 6404) in human. Leukocytes express L-selectin ligands that are critical for leukocyte-leukocyte interactions at sites of inflammation. PSGL-1 is a predominant leukocyte L-selectin ligand that displays appropriate sialyl Lewis x (sLex)-like carbohydrate determinants for L-selectin recognition. It is a single-pass, mucin-like type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce a form that contains an extracellular domain (18-320), a transmembrane domain (321-341), and a cytoplasmic domain (342-412). The mature form is generated following the cleaved of the propeptide (aa 18-41) region. PSGL-1 is a SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. It is constitutively expressed on all human peripheral-blood T cells and is also expressed on skin-homing T cells. Clone HECA-452 can detect a human endothelial cell differentiation antigen (HECA) selectively expressed on high endothelium. In addition to reacting with high endothelium, it also shows some cross-reactivity with a set of monocytic cells. This clone is shown to block the majority of P-selectin binding to neutrophils. (Ref.: Tu, L., et al. (1999). J. Immunol. 163(9); 5070-5078; Fuhlbrigge, RC., et al. (1997). Nature. 389(6654); 978-981; Duivestijn, AM., et al. (1988). Am. J. Pathol. 130(1); 147-155).
      References
      Product Information
      FormatPurified
      Control
      • MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
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      PresentationPurified rat monoclonal antibody IgM in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-PSGL-1/CD162, clone HECA-452, Cat. No. MABF3036, is a rat monoclonal antibody that detects P-selectin glycoprotein ligand 1 (PSGL-1) and is tested for use in Flow Cytometry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunohistochemistry
      Application NotesTested applications

      Immunohistochemistry Applications: A representative lot detected PSGL-1/CD162 in Immunohistochemistry application (Duijvestijn, A.M., et al. (1988). Am J Pathol. 130(1):147-55).

      Flow Cytometry Analysis: A representative lot detected PSGL-1/CD162 in Flow Cytometry application (Picker, L.J., et al. (1991). Nature. 349(6312):796-9; Fuhlbrigge, R.C., et al. (1997). Nature. 389(6654):978-81).

      Western Blotting Analysis: A representative lot detected PSGL-1/CD162 in Western Blotting application (Fuhlbrigge, R.C., et al. (1997). Nature. 389(6654):978-81).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenStromal preparation from human tonsil tissue.
      EpitopeExtracellular domain
      CloneHECA-452
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone HECA-452 is a rat monoclonal antibody that detects PSGL-1/CD162. It targets an epitope within the extracellular domain.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SELPLG
      Purification MethodLigatrap
      UniProt Number
      Molecular Weight43.2 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

      Flow Cytometry Analysis (FC): 1.0 µg of this antibody stained one million Human peripheral blood mononuclear cells (PBMC).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3036-100UG 04065269049135