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MABN688 Anti-PTH1R Antibody, clone 4D2

Anti-PTH1R Antibody, clone 4D2 is a highly specific mouse monoclonal antibody that targets PTHR1 and has been tested in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Anti-PTH1R Antibody, clone 4D2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: PFE, PTH1R, PTHR, PTHR1 UniProt Number: Q03431
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MABN688
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN688
      DescriptionAnti-PTH1R Antibody, clone 4D2
      Alternate Names
      • Parathyroid hormone 2 receptor
      • PTH2 receptor
      • PTH1R
      Background InformationParathyroid hormone-related peptide receptor (PTH1R) or alternatively known as PTH/PTHr receptor is encoded by the human gene named PTH1R/PTHR/PTHR1. The protein is the receptor for parathyroid hormone and for parathyroid hormone related peptide. It is a multi-pass G-protein coupled receptor localized to the cell membrane. It is expressed in most tissues but particularly in the kidney, bone and liver. The activity of the receptor is mediated through adenyl cyclase, phospholipase C, and phosphatidylinositol-calcium secondary messenger pathways. Parathyroid hormone related peptide regulates calcium ion homeostasis and when PTH1R is activated by ligand binding osteoblasts express RANKL which binds RANK on osteoclasts, which activates them to increase the resorption of calcium. Defects in PTH1R cause various defects in cartilage, bone, and tooth development as well as tumors of bone and cartilage. EMD-Millipore’s Anti-PTH1R mouse monoclonal antibody has been tested in western blot on transfected recombinant protein and in paraffin embedded immunohistochemistry on human kidney tissue sections and fluorescent immunocytochemistry on SK-BR-3 cells in culture.
      References
      Product Information
      FormatAscites
      Control
      • PTH1R-hIgGFc transfected HEK293 cell lysate
      PresentationMouse monoclonal IgG1 ascites fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PTH1R Antibody, clone 4D2 is a highly specific mouse monoclonal antibody that targets PTHR1 and has been tested in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected PTH1R in human kidney tissue.

      Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected PTH1R in SK-BR-3 cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human PTH1R expressed in E. coli.
      Clone4D2
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PFE
      • PTH1R
      • PTHR
      • PTHR1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~66 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PTH1R-hIgGFc transfected HEK293 cell lysate.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PTH1R-hIgGFc transfected HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN688 04053252917516

      Documentation

      Anti-PTH1R Antibody, clone 4D2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PTH1R Antibody, clone 4D2 Certificates of Analysis

      TitleLot Number
      Anti-PTH1R, clone 4D2 - VP1401287 VP1401287
      Anti-PTH1R, clone 4D2 - QVP1305165 QVP1305165
      Anti-PTH1R, clone 4D2 - VP2204070 VP2204070
      Anti-PTH1R, clone 4D2 - VP2405160 VP2405160
      Anti-PTH1R, clone 4D2 -VP1505071 VP1505071
      Anti-PTH1R, clone 4D2 -VP1509241 VP1509241
      Anti-PTH1R, clone 4D2 -VP1511205 VP1511205
      Anti-PTH1R, clone 4D2 -VP1607197 VP1607197
      Anti-PTH1R, clone 4D2 Monoclonal Antibody VP1805312
      Anti-PTH1R, clone 4D2 Monoclonal Antibody VP1710059
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