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MABS1923-25UG Anti-PTK7 Antibody, clone 31G9

Anti-PTK7, clone 31G9, Cat. No. MABS1923, is a rat monoclonal antibody that detects Inactive tyrosine-protein kinase 7 (PTK7) and has been tested for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-PTK7 Antibody, clone 31G9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PTK7, CCK4 UniProt Number: Q13308
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MABS1923-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HFC, IF, IP, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1923-25UG
      DescriptionAnti-PTK7 Antibody, clone 31G9
      Alternate Names
      • Inactive tyrosine-protein kinase 7
      • Colon carcinoma kinase 4
      • CCK-4
      • Protein-tyrosine kinase 7
      • Pseudo tyrosine kinase receptor 7
      • Tyrosine-protein kinase-like 7
      Background InformationInactive tyrosine-protein kinase 7 (UniProt: Q13308; also known as Colon carcinoma kinase 4, CCK-4, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7, PTK7) is encoded by the PTK7 (also known as CCK4) gene (Gene ID: 5754) in human. PTK7 is a single-pass type I membrane protein that lacks the catalytic activity. It is highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. It is weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Its expression is reported to be higher in cell lines established from normal non-tumorigenic tissues compared those from highly metastatic invasive carcinomas. Six isoforms of PTK7 have been described that are generated by alternative splicing. It is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate mature form. It contains seven Ig-like-C2 type domains. It has an extracellular domain (aa31-704), a transmembrane domain (aa 705-725; and a cytoplasmic domain (aa 726-1070). PTK7 is shown to associate with receptor tyrosine kinase-like orphan receptor 2 (ROR2) to form a heterodimeric complex in mammalian cells and PTK7 and ROR2 physically and functionally interact with the non-canonical WNT5A ligand leading to JNK activation and cell movements. PTK7 is cleaved by MMP14 between Pro 621 and Leu 622 to generate an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. (Ref.: Martinez, S., et al. (2015). J. Biol. Chem. 290(51); 30562-72).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PTK7, clone 31G9, Cat. No. MABS1923, is a rat monoclonal antibody that detects Inactive tyrosine-protein kinase 7 (PTK7) and has been tested for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A 1:25 dilution from a representative lot detected PTK7 in human skin and human placenta tissues.

      Immunofluorescence Analysis: A representative lot detected PTK7 in Immunofluorescence applications (Martinez, S., et. al. (2015). J Biol Chem. 290(51):30562-72).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated PTK7 in HEK293 cell lysate. (Martinez, S., et. al. (2015). J Biol Chem. 290(51):30562-72).

      Western Blotting Analysis: A representative lot detected PTK7 in Western Blotting applications (Martinez, S., et. al. (2015). J Biol Chem. 290(51):30562-72).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the extracellullar domain of human Inactive tyrosine-protein kinase 7 (PTK7).
      Epitopeextracellular domain
      Clone31G9
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 31G9 is a rat monoclonal antibody that detects human Inactive tyrosine protein kinase 7 (PTK7). It targets an epitope within the extracellular domain.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTK7
      • CCK4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight118.40 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in NCI-H460 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected PTK7 in one million NCI-H460 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1923-25UG 04054839448287