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MABF2050 Anti-Peripheral Node Addressin (PNAd) Antibody, clone MECA-79

This rat monoclonal Anti-Peripheral Node Addressin (PNAd) Antibody, clone MECA-79, Cat. No. MABF2050, is tested in Flow Cytometry, Immunohistochemistry (Paraffin), , Immunoprecipitation, Inhibition, and Western Blotting for the detection of Peripheral node addressin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology
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MABF2050
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HFC, IH(P), IP, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2050
      DescriptionAnti-Peripheral Node Addressin (PNAd) Antibody, clone MECA-79
      Alternate Names
      • Peripheral Node Addressin
      • MECA-79
      • CD62L-ligand (PNAd)
      Background InformationPeripheral lymph node addressin (PNAd) is a mixture of glycoproteins that is expressed on high endothelial venules (HEV) and is required for lymphocyte homing. It is shown to be a ligand for CD62L (L-selectin) that is expressed on the surface of naïve and central memory T cells. PNAd is a sulfated and fucosylated glycoprotein recognized by the prototypic monoclonal antibody, MECA-79. Naïve T cells use PNAd to traffic into lymphoid organs in non-mucosal tissue sites. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. Regulated appearance of PNAd+ HEVs is found during ontogeny of lymphoid tissues as well as in chronic inflammatory conditions. PNAd+ HEVs are reported to appear 24-48 h after birth in mouse peripheral lymph nodes and persist throughout life to support recirculation of lymphocytes. In humans PNAd+ HEVs are detected in fetal peripheral lymph nodes. PNAd+ HEVs are also shown to be present in several chronic inflammatory conditions, including thyroiditis, ulcerative colitis, psoriasis, rheumatoid synovitis, and cutaneous lymphomas. In murine models of inflammation, PNAd+ HEV-like vessels develop and these vessels are found to support lymphocyte recruitment. Studies on cancer in murine models and in humans have identified PNAd as a biomarker of improved disease prognosis and blockade of PNAd or its ligand, L-selectin, is shown to abrogate protective antitumor immunity in murine models. (Ref.: Sinha, RK et al. (2006). Vet. Immunol. Immunopathol. 110 (1-2): 97-108).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgM in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal Anti-Peripheral Node Addressin (PNAd) Antibody, clone MECA-79, Cat. No. MABF2050, is tested in Flow Cytometry, Immunohistochemistry (Paraffin), , Immunoprecipitation, Inhibition, and Western Blotting for the detection of Peripheral node addressin.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Peripheral Node Addressin (PNAd) in human liver and human pancreas tissues.

      Western Blotting Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Western Blotting applications (Hemmerich, S., et. al. (1994). J Exp Med. 180(6):2219-26).

      Inhibition Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Inhibition applications (Streeter, P.R., et. al. (1988). J Cell Biol. 107(5):1853-62;Michie, S.A., et. al. (1993). Am. J. Pathol. 143(6):1688-98; Berg, E.L., et. al. (1991). J Cell Biol. 114(2):343-9).

      Immunoprecipitation Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Immunoprecipitation applications (Berg, E.L., et. al. (1991). J Cell Biol. 114(2):343-9).

      Immunohistochemistry Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Immunohistochemistry applications (Sinha, R.K., et. al. (2006). Immunology. 119(4):461-9; Streeter, P.R., et. al. (1988). J Cell Biol. 107(5):1853-62; Michie, S.A., et. al. (1993). Am J Pathol. 143(6):1688-98).

      Flow Cytometry Analysis: A representative lot detected Peripheral Node Addressin (PNAd) in Flow Cytometry applications (Sinha, R.K., et. al. (2006). Immunology. 119(4):461-9).
      Biological Information
      ImmunogenCollagenase-dispersed lymph node stromal cells from BALB/c mice.
      CloneMECA-79
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone MECA-79 detects Peripheral Node Addressin (PNAd) in human and mouse.
      IsotypeIgMκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Molecular Weight50-250 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human tonsil tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Peripheral Node Addressin (PNAd) in human tonsil tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2050 04054839189180

      Documentation

      Anti-Peripheral Node Addressin (PNAd) Antibody, clone MECA-79 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Peripheral Node Addressin (PNAd) Antibody, clone MECA-79 Certificates of Analysis

      TitleLot Number
      Anti-Peripheral Node Addressin (PNAd), Clone MECA-79 - 4124921 4124921
      Anti-Peripheral Node Addressin (PNAd), Clone MECA-79 - 4231238 4231238
      Anti-Peripheral Node Addressin (PNAd), clone MECA-79 - 3393293 3393293
      Anti-Peripheral Node Addressin (PNAd), clone MECA-79 - 3761789 3761789
      Anti-Peripheral Node Addressin (PNAd), clone MECA-79 - 3882075 3882075
      Anti-Peripheral Node Addressin (PNAd), clone MECA-79 - 3988494 3988494
      Anti-Peripheral Node Addressin (PNAd), clone MECA-79 - 4044208 4044208
      Anti-Peripheral Node Addressin (PNAd), clone MECA-79 Monoclonal Antibody 3077877
      Anti-Peripheral Node Addressin (PNAd), clone MECA-79 Monoclonal Antibody Q2830942