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MABF2084-200UL Anti-Phosphorylcholine Antibody, clone BH8

Anti-Phosphorylcholine, clone BH8, Cat. No. MABF2084, is a mouse monoclonal antibody that detects Phosphorylcholine and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Inhibition/Function studies, and Western Blotting.

Anti-Phosphorylcholine Antibody, clone BH8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology
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MABF2084-200UL
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AELISA, FC, ICC, INHIB, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2084-200UL
      DescriptionAnti-Phosphorylcholine Antibody, clone BH8
      Alternate Names
      • PC
      • ChoP
      Background InformationPhosphorylcholine (PC) is considered as an important immunodominant determinant of pneumococcal teichoic acids. It is found in the cell walls of a variety of pathogenic and nonpathogenic microorganisms, including Streptococcus pneumoniae, lactobacillus, certain fungi, and nematodes. PC plays an important protective role in pneumococcal bacterial infection. It is also a major prerequisite for proinflammatory effects of PAF and PAF-like lipids.Antibodies to PC are naturally present in humans and their levels are reported to decline with age. Natural PC-specific antibodies are reported to constitute about 5 to 10% of the total IgM pool in humans. Antibodies to PC have been subdivided into two populations, Group I and II, based on their affinity for PC and p-nitrophenyl phosphorylcholine (NPPC). Group I anti-PCs bind both PC and NPCC whereas Group II require a phenyl group and bind only to NPPC. Anti-PC IgM, IgA, and IgG1 belong to the Group I and Anti-PC IgG2 is entirely made up of Group II antibodies. Anti-PC IgM has are reported to exhibit a negative correlation with atherosclerosis development in hypertensive individuals and low levels of anti-PC may be used to predict development of cardiovascular disease. (Ref.: Su, J., et al. (2006). Atherosclerosis. 188(1):160-166; Sjoberg, BG et al. (2009). Atherosclerosis. 203(2); 528-532).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS without azide.
      Applications
      ApplicationAnti-Phosphorylcholine, clone BH8, Cat. No. MABF2084, is a mouse monoclonal antibody that detects Phosphorylcholine and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Inhibition/Function studies, and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Inhibits Activity/Function
      • Western Blotting
      Application NotesELISA Analysis: A representative lot detected Phosphorylcholine in ELISA applications (Patel, P.S., et. al. (2015). J. Immunol. 194(12):5838-50).

      Inhibits Activity/Function Analysis: A representative lot inhibited lung-resident APC activation and diminished the infiltration of allergy-associated cells into the pulmonary parenchyma in mice. (Patel, P.S., et. al. (2015). J Immunol. 194(12):5838-50).


      ELISA Analysis: A representative lot detected Phosphorylcholine in ELISA applications (Ansel, K.M., et. al. (2002). Immunity. 16(1):67-76).

      Flow Cytometry Analysis: A representative lot detected Phosphorylcholine in Flow Cytometry applications (Patel, P.S., et. al. (2015). J. Immunol. 194(12):5838-50).

      Immunocytochemistry Analysis: A representative lot detected Phosphorylcholine in Immunocytochemistry applications (Patel, P.S., et. al. (2015). J. Immunol. 194(12):5838-50).
      Biological Information
      ImmunogenBacteria (Streptococcus pneumoniae, non-encapsulated strain R36A).
      CloneBH8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone BH8 speifically detects phosphocholine conjugated to BSA.
      IsotypeIgMκ
      Species Reactivity
      • All
      Species Reactivity NoteAll.
      Antibody TypeMonoclonal Antibody
      Molecular Weight~66 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Phosphorylcholine-BSA conjugate.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected Phosphorylcholine-BSA conjugate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2084-200UL 04054839460210

      Documentation

      Anti-Phosphorylcholine Antibody, clone BH8 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Phosphorylcholine Antibody, clone BH8 Certificates of Analysis

      TitleLot Number
      Anti-Phosphorylcholine, clone BH8 - 3449076 3449076
      Anti-Phosphorylcholine, clone BH8 - 3892593 3892593
      Anti-Phosphorylcholine, clone BH8 - 4011222 4011222
      Anti-Phosphorylcholine, clone BH8 - 4048675 4048675
      Anti-Phosphorylcholine, clone BH8 - 4131192 4131192
      Anti-Phosphorylcholine, clone BH8 - 4235326 4235326
      Anti-Phosphorylcholine, clone BH8 - 4328284 4328284
      Anti-Phosphorylcholine, clone BH8 Monoclonal Antibody Q3019649