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MABS1327-25UL Anti-Podocalyxin (GP135) Antibody, clone 3F2:D8

Anti-Podocalyxin (GP135), clone 3F2:D8, Cat. No. MABS1327, is a mouse monoclonal antibody that detects Podocalyxin (GP135) and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PODXL, PCLP, PCLP1 UniProt Number: Q52S86
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MABS1327-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      CaICC, IF, IP, RIA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1327-25UL
      DescriptionAnti-Podocalyxin (GP135) Antibody, clone 3F2:D8
      Alternate Names
      • Podocalyxin-like protein 1
      • GP1345
      • PC
      • PC-like protein 1
      • PCLP-1
      • cPCLP1
      Background InformationPodocalyxin (UniProt: Q52S86; also known as Gp135, Podocalyxin-like protein 1, PC, PC-like protein 1, PCLP-1, cPCLP1) is encoded by the PODXL (also known as PCLP, PCLP1) gene (Gene ID: 482252) in canine species. GP135 is an apical membrane protein expressed in polarized MDCK epithelial cells. Some expression is also observed in heart, lung, renal cortex and medulla, and muscle. It has an extracellular domain (aa 23-474), a helical domain (aa 475-495) and a cytoplasmic domain (aa 496-571). Its cytoplasmic domain is shown to be essential for the apical membrane targeting and renal tubulogenesis. GP135 is involved in the regulation of both adhesion and cell morphology and cancer progression. It functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. It can also act as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands and thereby increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Gp135 is also reported to be involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. It co-localizes with actin filaments, ezrin and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Gp135 is shown to undergo N- and O-linked glycosylation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Podocalyxin (GP135), clone 3F2:D8, Cat. No. MABS1327, is a mouse monoclonal antibody that detects Podocalyxin (GP135) and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected Podocalyxin (GP135) in MDCK treated with iodoacetamid cell lysate.

      Radioimmunoassay Analysis: A representative lot detected Podocalyxin (GP135) in Radioimmunoassay applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87).

      Immunofluorescence Analysis: A representative lot detected Podocalyxin (GP135) in Immunofluorescence applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87; Cheng, H.Y., et. al. (2005). J Am Soc Nephrol. 16(6):1612-22).

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Podocalyxin (GP135) in MDCK cells (Courtesy of Dr. Tzuu-Shuh Jou, M.D., Ph.D., Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University).

      Immunocytochemistry Analysis: A representative lot detected Podocalyxin (GP135) in Immunocytochemistry applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87; Cheng, H.Y., et. al. (2005). J Am Soc Nephrol. 16(6):1612-22).

      Western Blotting Analysis: A representative lot detected Podocalyxin (GP135) in Western Blotting applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87; Cheng, H.Y., et. al. (2005). J Am Soc Nephrol. 16(6):1612-22).

      Immunoprecipitation Analysis: A representative lot detected Podocalyxin (GP135) in Immunoprecipitation applications (Ojakian, G.K., et. al. (1988). J Cell Biol. 107(6 Pt 1):2377-87; Cheng, H.Y., et. al. (2005). J Am Soc Nephrol. 16(6):1612-22).
      Biological Information
      ImmunogenIntact Madin-Darby canine kidney (MDCK) cells.
      Clone3F2:D8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3F2:D8 detects Podocalyxin in Madin-Darby canine kidney (MDCK) cells.
      IsotypeIgG1κ
      Species Reactivity
      • Canine
      Species Reactivity NoteCanine.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PODXL
      • PCLP
      • PCLP1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight180 kda obseerved; 59.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MDCK cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Podocalyxin (GP135) in MDCK cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1327-25UL 04054839522109