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GR18 Anti-Progesterone Receptor (Ab-1) Mouse mAb (PR4-12)

This Anti-Progesterone Receptor (Ab-1) Mouse mAb (PR4-12) is validated for use in Immunoblotting, Paraffin Sections for the detection of Progesterone Receptor (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PR

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GR18
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Ch, Fe, H, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~94 kDa progesterone receptor A and the ~120 kDa progesterone receptor B in T47D cells and human breast carcinoma tissue.
      Catalogue NumberGR18
      Brand Family Calbiochem®
      SynonymsAnti-PR
      References
      ReferencesMenendez-Botet, C.J. and Schwartz, M.K. J. 1992. Int. Fed. Clin. Chem. 4, 94.
      Clarke, R., et al. 1991. Nuclear Hormone Receptors, 297, Academic Press, New York.
      Green, S. and Chambon, P. 1991. Nuclear Hormone Receptors., 15, Academic Press, New York.
      Wahli, W. and Martinez, E. 1991. FASEB J. 5, 2243.
      Beato, M. 1989. Cell 56, 335.
      Evans, R.M. 1988 Science 240, 889.
      Wittliff, J.L. 1988. in Cancer of the Breast. (Donegan, W. L. and Spratt, J.S. eds. et al.) p303. Saunders Philadelphia.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlHBL 100 cells
      Positive controlT-47D cells or human breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1-5 µg/ml)
      Paraffin Sections (0.5-1 µg/ml, see comments)
      Application CommentsNo pre-treatment required for staining human paraffin sections but using the pressure cooker method may enhance staining. Trypsin is required for rat or chicken paraffin sections. Trypsin/saponin is required for feline paraffin sections. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human progesterone receptor
      ImmunogenHuman
      ClonePR4-12
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Chicken
      • Feline
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR18 0

      Documentation

      Anti-Progesterone Receptor (Ab-1) Mouse mAb (PR4-12) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Progesterone Receptor (Ab-1) Mouse mAb (PR4-12) Certificates of Analysis

      TitleLot Number
      GR18

      References

      Reference overview
      Menendez-Botet, C.J. and Schwartz, M.K. J. 1992. Int. Fed. Clin. Chem. 4, 94.
      Clarke, R., et al. 1991. Nuclear Hormone Receptors, 297, Academic Press, New York.
      Green, S. and Chambon, P. 1991. Nuclear Hormone Receptors., 15, Academic Press, New York.
      Wahli, W. and Martinez, E. 1991. FASEB J. 5, 2243.
      Beato, M. 1989. Cell 56, 335.
      Evans, R.M. 1988 Science 240, 889.
      Wittliff, J.L. 1988. in Cancer of the Breast. (Donegan, W. L. and Spratt, J.S. eds. et al.) p303. Saunders Philadelphia.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-October-2007 JSW
      SynonymsAnti-PR
      ApplicationImmunoblotting (1-5 µg/ml)
      Paraffin Sections (0.5-1 µg/ml, see comments)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~94 kDa progesteron receptor A and the ~120 kDa progesterone receptor B.
      BackgroundThe progesterone receptor (PR) is a member of the superfamily of steroid hormone receptors. In response to ligand binding, these nuclear receptors bind to their target DNA response element and modulate the transcription of specific genes. Hormone induced binding of receptor to its response element promotes the formation of a stable initiation complex, which allows for efficient transcription by RNA polymerase II. Steroid hormone receptors are characterized by a six domain structure labeled A through F. The A/B domain is responsible for trans-activation, C is the DNA binding region and the most highly conserved, D is involved in nuclear localization and E has a variety of functions including ligand binding, dimerization, interaction with HSP90 and trans-activation. There are two forms of the progesterone receptor; the A-receptor (~94 kDa) is a naturally occurring, truncated form of the B-receptor (~120 kDa). Both forms bind progesterone but may have distinct biological properties. PR is expressed in several tissues especially uterus and breast. PR is one of the most important hormones in the regulation of growth and differentiation in mammary tissue and is thought to play an important role in mammary carcinogenesis. The measurement of PR has become an important prognostic factor in breast cancer and is useful for predicting the likelihood of response to endocrine therapy.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human progesterone receptor
      ClonePR4-12
      IsotypeIgG₁
      Specieschicken, feline, human, rat
      Positive controlT-47D cells or human breast carcinoma tissue
      Negative controlHBL 100 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsNo pre-treatment required for staining human paraffin sections but using the pressure cooker method may enhance staining. Trypsin is required for rat or chicken paraffin sections. Trypsin/saponin is required for feline paraffin sections. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesMenendez-Botet, C.J. and Schwartz, M.K. J. 1992. Int. Fed. Clin. Chem. 4, 94.
      Clarke, R., et al. 1991. Nuclear Hormone Receptors, 297, Academic Press, New York.
      Green, S. and Chambon, P. 1991. Nuclear Hormone Receptors., 15, Academic Press, New York.
      Wahli, W. and Martinez, E. 1991. FASEB J. 5, 2243.
      Beato, M. 1989. Cell 56, 335.
      Evans, R.M. 1988 Science 240, 889.
      Wittliff, J.L. 1988. in Cancer of the Breast. (Donegan, W. L. and Spratt, J.S. eds. et al.) p303. Saunders Philadelphia.