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MABE341 Anti-Puromycin Antibody, clone 17H1

Anti-Puromycin antibody, clone 17H1 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.

Anti-Puromycin Antibody, clone 17H1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins
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MABE341
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, AWBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE341
      DescriptionAnti-Puromycin Antibody, clone 17H1
      Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Puromycin antibody, clone 17H1 detects puromycin incorporated into protein. Monoclonal antibodies to puromycin may also be used with standard immunochemical methods.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPuromycin from Streptomyces alboniger
      Clone17H1
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • All
      Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightPuromycin is incorporated in neosynthesized proteins. In the presense of Puromycin only, this antibody detects Puromycin-incorporated neosynthesized proteins at multiple molecular weights. However, a decrease in signal is observed in the co-presense of Cycloheximide, an inhibitor of protein biosynthesis in eukaryotic organisms.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysates treated with Puromycin and Cyclohexamide, or with Puromycin only.

      Western Blotting Analysis: A 1:100,000 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HEK293 cell lysates treated with Puromycin only. This antibody also detected small mounts of Puromycin-incorporated neosynthesized proteins in HEK293 cells treated with Puromycin and Cyclohexamide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE341 04053252909214

      Documentation

      Anti-Puromycin Antibody, clone 17H1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Puromycin Antibody, clone 17H1 Certificates of Analysis

      TitleLot Number
      Anti-Puromycin, clone 17H1 3082497
      Anti-Puromycin, clone 17H1 - 3194622 3194622
      Anti-Puromycin, clone 17H1 - 3258755 3258755
      Anti-Puromycin, clone 17H1 - 3403489 3403489
      Anti-Puromycin, clone 17H1 - 3600708 3600708
      Anti-Puromycin, clone 17H1 - 3808797 3808797
      Anti-Puromycin, clone 17H1 - 3928038 3928038
      Anti-Puromycin, clone 17H1 - 3948852 3948852
      Anti-Puromycin, clone 17H1 - 4093161 4093161
      Anti-Puromycin, clone 17H1 - 4215025 4215025
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