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05-952-I-25UG Anti-RNA Polymerase II Antibody, CTD Antibody, clone 8WG16

Anti-RNA Polymerase II, CTD, clone 8WG16, Cat. No. 05-952-I, is a mouse monoclonal antibody that detects DNA-directed RNA polymerase II subunit RPB1 and has been tested for use in Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Inhibition assay, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: POLR2A, POLR2 UniProt Number: P24928
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05-952-I-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, YeastDB, IP, Inhibition, WB, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-952-I-25UG
      DescriptionAnti-RNA Polymerase II Antibody, CTD Antibody, clone 8WG16
      Alternate Names
      • DNA-directed RNA polymerase II subunit RPB1
      • EC: 2.7.7.6
      • RNA polymerase II subunit B1
      • DNA-directed RNA polymerase II subunit A
      • DNA-directed RNA polymerase III largest subunit
      • RNA-directed RNA polymerase II subunit RPB1
      Background InformationDNA-directed RNA polymerase II subunit RPB1 (UniProt: P24928; also known as EC: 2.7.7.6, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, is encoded by the POLR2A (also known as POLR2) gene (Gene ID: 5430) in human. DNA-dependent RNA polymerase II catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RPB1 subunit is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that suggested to grab the incoming DNA template. It has multiple zinc and magnesium ion binding sites and contains a repetitive C-terminal domain (CTD; aa 1593-1960) that consists of tandem repeats of the consensus sequence YSPTSPS and serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. It can exist in both hypophosphorylated form, which is mainly cytosolic, and in hyperphosphorylated form. The phosphorylation of Serine 2 and 5 in the hepatapeptide repeat is achieved by Cdk7 and Cdk9. Cdk7 phosphorylation promotes transcription initiation by triggering promoter release and elongation.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without preservatives.
      Applications
      ApplicationAnti-RNA Polymerase II, CTD, clone 8WG16, Cat. No. 05-952-I, is a mouse monoclonal antibody that detects DNA-directed RNA polymerase II subunit RPB1 and has been tested for use in Dot Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Inhibition assay, and Western Blotting.
      Key Applications
      • Dot Blot
      • Immunoprecipitation
      • Inhibition
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: A representative lot detected RNA Polymerase II, CTD in Western Blotting applications (Patturajan, M., et. al. (1998). J Biol Chem. 273(8):4689-94; Zhang, J., et. al. (1991). J Biol Chem. 266(4):2290-6).

      Dot Blot Analysis: A representative lot detected RNA Polymerase II, CTD in Dot Blot applications (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).

      Immunoprecipitation Analysis: A representative immunoprecipitated RNA Polymerase II, CTD in Immunoprecipitation applications (Bhaumik, S.R., et. al. (2001). Genes Dev. 15(15):1935-45).

      Inhibition Analysis: A representative lot inhibited the interaction of RNA Polymerase II, CTD with the transcription complex. (Thompson, N.E., et. al. (1989). J Biol Chem. 264(19):11511-20).
      Biological Information
      ImmunogenRNA Polymerase II from wheat germ extract
      Clone8WG16
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8WG16 specifically detects DNA-directed RNA polymerase II subunit RPB1.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Yeast
      Species Reactivity NoteHuman, Yeast and almost all eukaryotic species, which has a concensus hepatapeptide repeat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • POLR2A
      • POLR2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~220 kDa observed; 217.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract cell lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected RNA Polymerase II, CTD in HeLa cell nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-952-I-25UG 04054839563270