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MABN2510-100UL Anti-RNF213 Antibody, clone 1C9

Anti-RNF213, clone 1C9, Cat. No. MABN2510, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase (RNF213) and is tested for use in ELISA, Immunocytochemistry, and Western Blotting.

MABN2510-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2510-100UL
      DescriptionAnti-RNF213 Antibody, clone 1C9
      Alternate Names
      • E3 ubiquitin-protein ligase RNF213
      • EC:2.3.2.27
      • ALK lymphoma oligomerization partner on chromosome 17
      • Mysterin
      • RING finger protein 213
      • RING-type E3 ubiquitin transferase RNF213
      Background InformationE3 ubiquitin-protein ligase RNF213 (UniProt: Q63HN8; also known as EC:2.3.2.27, ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RING-type E3 ubiquitin transferase RNF213) is encoded by the RNF213 (also known as ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR) gene (Gene ID: 57674) in human. RNF213 is a widely expressed, homooligomeric cytosolic protein that is involved in the non-canonical Wnt signaling pathway in vascular development. It acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, which leads to the inhibition of the non-canonical Wnt signaling pathway and promotes vessel regression. Its activity is induced by pro-inflammatory cytokines. RNF213 contains a Zinc finger domain (aa 3997-4036), which is required for its ubiquitin-protein ligase activity. It also contains an AAA domain, which is associated with ATPase activity. Four isoforms of RNF213 have been described that are produced by alternative splicing. Mutations in RNF213 gene have been linked to Moyamoya disease 2 (MYMY2) disease, a progressive cerebral angiopathy that can lead to transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-RNF213, clone 1C9, Cat. No. MABN2510, is a mouse monoclonal antibody that detects E3 ubiquitin-protein ligase (RNF213) and is tested for use in ELISA, Immunocytochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected RNF213 in Western Blotting applications (Kotani, Y., et. al. (2017). Sci Rep. 7:44293).

      ELISA Analysis: A representative lot detected RNF213 in Western Blotting applications (Kotani, Y., et. al. (2017). Sci Rep. 7:44293).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFLAG-tagged recombinant protein corresponding to the first 349 amino acids from the N-terminal region of human RNF213.
      EpitopeN-terminal
      Clone1C9
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1C9 is a mouse monoclonal antibody that detects human E3 ubiquitin-protein ligase (RNF213). It targets an epitope within the N-terminal domain.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNF213
      • ALO17
      • C17orf27
      • KIAA1554
      • KIAA1618
      • MYSTR
      Purification MethodLigatrap
      UniProt Number
      Molecular Weight591.41 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected RNF213 in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2510-100UL 04065265324342