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MABE2005-100UG Anti-RNF4 Antibody, clone 11-3.1

Anti-RNF4, clone 11-3.1, Cat. No. MABE2005, is a mouse monoclonal antibody that detects RNF4 and is tested for use in Western Blotting and Immunocytochemistry.

MABE2005-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE2005-100UG
      DescriptionAnti-RNF4 Antibody, clone 11-3.1
      Alternate Names
      • E3 ubiquitin-protein ligase RNF4
      • RING finger protein 4
      • Small nuclear ring finger protein
      • Protein SNURF
      Background InformationE3 ubiquitin-protein ligase RNF4 (UniProt: P78317; also known as RING finger protein 4, EC:2.3.2.27, Small nuclear ring finger protein, Protein SNURF) is encoded by the RNF4 (also known as SNURF) gene (Gene ID: 6047) in human. RNF4 gene encodes ubiquitin E3 ligase, an enzyme widely expressed in many tissues. RNF4 is mapped to human chromosome 4p16.3. The protein contains a RING-type zinc finger domain and around four small ubiquitin-related modifier (SUMO)-interaction motifs. The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates and the SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate. RNF4 is predominantly localized in the nucleus but can also be found in the cytoplasm. This dual localization allows it to function in multiple cellular compartments, dealing with SUMOylated substrates wherever they are located. The movement between these compartments can be regulated by specific signals or stress conditions. RNF4 is broadly expressed across various tissues, with notable levels in tissues that have high cellular turnover or are particularly responsive to stress, such as in immune cells and testis. It is involved in DNA repair, in transcription and chromatin regulation. It is also associated with the process of hematopoiesis. RNF4 is implicated in acute promyelocytic leukemia, acute myeloid leukemia, Fanconi anemia and aplastic anemia. Its role in maintaining protein homeostasis and responding to cellular stress makes it a key player in health and disease, with its dysfunction being linked to cancer, neurodegeneration, and developmental issues. Understanding the mechanisms regulating RNF4 activity and localization can provide insights into therapeutic strategies for these diseases. (Ref.: Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8. (PMID: 24695317).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-RNF4, clone 11-3.1, Cat. No. MABE2005, is a mouse monoclonal antibody that detects RNF4 and is tested for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesTested Applications

      Immunocytochemistry Analysis: A representative lot detected RNF4 in Immunocytochemistry application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).

      Western Blotting Analysis: A representative lot detected RNF4 in Western Blotting application (Saito, M., et al. (2014). Biochem Biophys Res Commun. 447(1):83-8).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged, recombinant human RNF4 protein.
      EpitopeUnknown
      Clone11-3.1
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 11-3.1 is a mouse monoclonal antibody that specifically detects recombinant human RNF4 protein.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNF4
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~ 32 kDa observed; 21.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution from a representative lot detected RNF4 protein in lysate from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE2005-100UG 04065272074032