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ABS437 Anti-RSPO1 Antibody

Detect R-spondin-1 using this rabbit polyclonal antibody, Anti-RSPO1 Antibody validated for use in western blotting, IHC & Immunofluorescence.

Anti-RSPO1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: RSPO1 UniProt Number: Q2MKA7
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ABS437
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IHC, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS437
      DescriptionAnti-RSPO1 Antibody
      Alternate Names
      • R-spondin1
      • RSPO
      • CRISTIN3
      • Roof plate-specific spondin-1
      • hRspo1
      Background InformationR-spondin-1 (RSPO1), or Roof plate-specific spondin-1 protein, is potent activator of the Wnt/beta-catenin signaling pathway leading to TCF/LEF (T cell-specific transcription factor/lymphoid enhancer factor) gene activation. RSPO1 operates on both the canonical Wnt/beta-catenin and non-canonical Wnt signaling pathways by acting as an inhibitor of ZNRF3 (zinc and ring finger 3) protein, a cell surface transmembrane E3 ubiquitin ligase, which is an important regulator of Wnt signaling. RSPO1 interacts with the extracellular domain of ZNRF3 and induces ZNRF3 membrane clearance. RSPO1 is also part of a family of secreted proteins that play critical roles in vertebrate development, including female sex development, and RSPO1 is required for early development of the gonads regardless of sex. Molecularly, RSPO1’s activities synergize with those of WNT4 and act to stabilize beta catenin which then activates its downstream targets. RSPO1 has been shown to be a potent mitogen for specific cell types such as gastrointestinal epithelium, and it is being examined as a key target for colon cancer therapy and for the treatment of mucositis (inflammation of the oral cavity, which is common among patients receiving chemotherapy or radiation for head and neck cancers). Recently RSPO1 was also shown to be a novel beta-cell line growth factor and a factor that promotes insulin secretion in a glucose independent fashion, thus making it a target for enhancing beta-cell function in patients with type 2 diabetes. Defects in RSPO1 can lead to female to male sex reversal and have been associated with the genetic disease PKKSCC (palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal). EMD Millipore’s Anti-RSPO1 rabbit polyclonal antibody has been tested in western blotting, immunohistochemistry, and immunofluorescence in human and rat samples.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rat spleen tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect R-spondin-1 using this rabbit polyclonal antibody, Anti-RSPO1 Antibody validated for use in western blotting, IHC & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected RSPO1 in human spleen tissue.

      Immunofluorescence Analysis: 20 μg/mL from a representative lot detected RSPO1 in human spleen cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human RSPO1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • RSPO1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~37 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms at ~22 kDa, ~26 kDa, and ~29 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat spleen tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected RSPO1 in 15 µg of rat spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS437 04053252905582