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DR1115 Anti-RUNX3 Rabbit pAb

Anti-RUNX3 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DR1115
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the 50 kDa RUNX3 protein.
      Catalogue NumberDR1115
      Brand Family Calbiochem®
      Application Data
      Detection of human RUNX3 by immunoprecipitation followed by immunoblotting. Whole cell lysate from cells transfected with RUNX3. Antibody for IP: Anti-RUNX3 Rabbit pAb (Cat. No. DR1115) (ratio of Ab to protein A beads 1:50-1:100) Antibody for IB: Anti-RUNX3 Mouse pAb. Detection: chemiluminescence.
      References
      Product Information
      Formliquid
      FormulationUndiluted serum.
      PreservativeNone
      Applications
      Key Applications Immunoprecipitation
      Application NotesImmunoprecipitation (see comments)
      Application CommentsFor immunoprecipitation the recomended ratio of antibody to protein A beads is 1:50-1:100, the dilution antibody must be determined for each sample. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, human RUNX3 (aa 1-429)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1115 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      ApplicationImmunoprecipitation (see comments)
      Application Data
      Detection of human RUNX3 by immunoprecipitation followed by immunoblotting. Whole cell lysate from cells transfected with RUNX3. Antibody for IP: Anti-RUNX3 Rabbit pAb (Cat. No. DR1115) (ratio of Ab to protein A beads 1:50-1:100) Antibody for IB: Anti-RUNX3 Mouse pAb. Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the 50 kDa RUNX3 protein.
      BackgroundThis protein is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.
      HostRabbit
      Immunogen speciesHuman
      ImmunogenFull-length, human RUNX3 (aa 1-429)
      IsotypeIgG
      Specieshuman
      Formliquid
      FormulationUndiluted serum.
      PreservativeNone
      CommentsFor immunoprecipitation the recomended ratio of antibody to protein A beads is 1:50-1:100, the dilution antibody must be determined for each sample. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review