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MABF2073-25UG Anti-Rabies Virus CVS-11 Antibody, clone 509-6

Anti-Rabies Virus CVS-11 , clone 509-6 Antibody, Cat. No. MABF2073-25UG, validated for use in Flow Cytometry and ELISA, Neutralizing and Fluorescence Activated Cell Sorting (FACS).

Research Category: Epigenetics & Nuclear Function Gene Symbol: G UniProt Number: O92284
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MABF2073-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VirusFC, ELISA, NEUT, FACSMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2073-25UG
      DescriptionAnti-Rabies Virus CVS-11 Antibody, clone 509-6
      Alternate Names
      • Glycoprotein
      • Glycoprotein Rabies virus
      • Rabies virus strain CVS-11
      Background InformationGlycoprotein (UniProt: O92284) is encoded by the G gene in Rabies virus, strain CVS-11. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane.  The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface.   Clone 509-6 is shown to neutralize all fixed and street rabies virus strains except some virus isolates from bats. The conformational antigenic site I for this clone is located at aa 231. (Ref.: Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et al (2005). J. Virol. 79(8): 4672–4678).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Rabies Virus CVS-11 , clone 509-6 Antibody, Cat. No. MABF2073-25UG, validated for use in Flow Cytometry and ELISA, Neutralizing and Fluorescence Activated Cell Sorting (FACS).
      Key Applications
      • Flow Cytometry
      • ELISA
      • Neutralizing
      • Fluorescence Activated Cell Sorting (FACS)
      Application NotesNeutralizing Analysis: A representative lot neutralized Rabies Virus (Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5; Bunschoten, H., et. al. (1989). J Gen. Virol. 70 ( Pt 2):291-8; Marissen, W.E., et. al. (2005). J Virol. 79(8):4672-8).
      Biological Information
      ImmunogenBeta-propiolactone inactivated Rabies virus, strain CVS-11 (RABV).
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 509-6 detected glycoprotein from Rabies virus, strain CVS-11 in L929 cells expressing the rabies protein. It did not detect glycoprotein in wild type L929 cells.
      IsotypeIgG2a
      Species Reactivity
      • Virus
      Species Reactivity NoteRabies Virus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • G
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in L929 cells expressing the rabies protein and in L929 (WT) cells. Flow Cytometry Analysis: 1 µg of this antibody detected glycoprotein of Rabies virus in one million L929 cells expressing the rabies protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2073-25UG 04054839421105

      Documentation