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05-530-I Anti-Rad51 Antibody, clone 3C10

This Anti-Rad51 Antibody, clone 3C10 is validated for use in Western Blotting, Immunoprecipitation for the detection of Rad51 .

Anti-Rad51 Antibody, clone 3C10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: RAD51, RAD51A, RECA UniProt Number: Q06609
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05-530-I
200 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-530-I
      Replaces05-530
      DescriptionAnti-Rad51 Antibody, clone 3C10
      Alternate Names
      • DNA repair protein RAD51 homolog 1
      • HsRAD51
      • hRAD51
      • RAD51 homolog A
      • Rad51
      Background InformationDNA repair protein RAD51 homolog 1 (UniProt Q06609; also known as BRCA1/BRCA2-containing complex subunit 5, Homolog of E. coli RecA, hRAD51, HsRAD51, RAD51 homolog A, RecA-like protein, Recombination protein A) is encoded by the RAD51 (also known as HRAD51, HsT16930, BRCC5, MRMV2, RAD51A, RECA) gene (Gene ID 5888) in human. RAD51 is a member of the RecA family recombinases. It interacts with BRCA1 and BRCA2 to activate homologous recombination. RAD51 exhibits DNA-dependent ATPase activity and participates in double-strand break (DSB) repair by unwinding DNA to form a helical nucleoprotein filament. It is highly expressed in the testis and thymus and lower expression levels are found in the small intestine, placenta, colon, pancreas, and ovaries.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Rad51 Antibody, clone 3C10 is validated for use in Western Blotting, Immunoprecipitation for the detection of Rad51 .
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: Reported by an independent laboratory to immunoprecipitate.
      Biological Information
      ImmunogenRecombinant protein corresponding to human Rad51.
      Clone3C10
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RAD51
      • RAD51A
      • RECA
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~37 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rad51 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-530-I 04055977173369

      Documentation

      Anti-Rad51 Antibody, clone 3C10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Rad51 Antibody, clone 3C10 Certificates of Analysis

      TitleLot Number
      Anti-Rad51, clone 3C10 (mouse monoclonal) Monoclonal Antibody Q2922327
      Anti-Rad51, clone 3C10 - 3436195 3436195
      Anti-Rad51, clone 3C10 - 3832627 3832627
      Anti-Rad51, clone 3C10 - 3931783 3931783
      Anti-Rad51, clone 3C10 - 4137149 4137149
      Anti-Rad51, clone 3C10 - 4149123 4149123
      Anti-Rad51, clone 3C10 -Q2574784 Q2574784

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies