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AB9728 Anti-S100A12 Antibody

AB9728
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, IHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9728
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-S100A12 Antibody
      Alternate Names
      • EN-RAGE
      • Extacellular newly identified RAGE-binding protein
      • CAAF1
      • Calcium-binding protein in amniotic fluid-1
      • Calgranulin C
      • Calgranulin-related protein
      • CGRP
      • Cornea-associated antigen
      • CO-Ag
      Background InformationS100 calcium binding protein A12, also known as S100A12, EN-RAGE, and calgranulin C, is a proinflammatory protein with significant potential as a biomarker. It is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. The member S100A12 is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. It has been reported to be secreted by activated granulocytes and binds to the receptor for advanced gycation end products, which induces NfkappaB-dependent activation of endothelium (Foell et al., 2003). S100A12 is strongly expressed in inflamed synovial tissue typical in rheumatoid and psoriatic arthritis and may also prove an excellent biomarker for Inflammatory bowel disease (Kaiser et al., 2007). S100A12 protein may also be involved in chronic inflammation in atherosclerotic lesions including those of Type II diabetics (Kosaki et al. 2004) and the acceleration of atherosclerosis in hemodialysis patients (Mori et al., 2009).

      Foell, D. et al. (2003). Expression of the pro-inflammatory protein S100A12 (EN-RAGE) in rheumatoid and psoriatic arthritis. Rheumatol. 42:1383-1389.
      Kaiser, T. et al. (2007). Faecal S100A12 as a non-invasive marker distinguishing inflammatory bowel disease from irritable bowel syndrome. Gut. 56:1706-1713.
      Kosaki, A. et al. (2004). Increased plasma S100A12 (EN-RAGE) levels in patients with type 2 diabetes. J. Clin. Endocrinol. Metabol. 89:5423-5428.
      Mori, Y. et al. (2009). Increased Plasma S100A12 (EN-RAGE) levels in hemodialysis patients with atherosclerosis. Am. J. Nephrol. 29:18-24.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HL-60 cells, and human PMN/Granulocytes {Vogl, T et al (1999) “S100A12 Is Expressed Exclusively by Granulocytes and Acts Independently from MRP8 and MRP14” J. Biol. Chem. 274(3):25291-25296}.
      PresentationLiquid in PBS with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-S100A12 Antibody is validated for use in ELISA, IH, WB for the detection of S100A12.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:1,000-1:10,000 on recombinant human S100A12.
      Immunohistochemistry: fesh frozen, acetone fixed sections 1:100-1:500 using ABC enzymatic detection methods (Srikrishna, G. et al, 2005). Other fixation conditions not yet tested.
      ELISA: 1:10,000-1:100,000 against the recombinant human S100A12.
      It is expected that the antibody will also work for immunohistochemistry.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenFull length recombinant His-tagged human S100A12 that was expressed in E. coli and purified by Ni-NTA affinity chromatography prior to immunization.
      HostRabbit
      SpecificityBy western blot this polyclonal antibody recognizes a band at ~10-11 kDa under reducing conditions. A dimer can be sometimes be seen at ~ 21 kDa using recombinant human S100A12. Antibody was affinity purifed over immobilized S100A12 prior to vialing.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-S100A12 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-S100A12 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-S100A12 -
      RABBIT ANTI-S100A12 - 3222607 3222607
      RABBIT ANTI-S100A12 - 3497311 3497311
      RABBIT ANTI-S100A12 - 3708267 3708267
      RABBIT ANTI-S100A12 -2527060 2527060
      RABBIT ANTI-S100A12 -2632687 2632687
      RABBIT ANTI-S100A12 -2733749 2733749
      RABBIT ANTI-S100A12 -2768409 2768409
      RABBIT ANTI-S100A12 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2007331 2007331
      RABBIT ANTI-S100A12 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2224433 2224433

      References

      Reference overviewSpeciesPub Med ID
      Carboxylated glycans mediate colitis through activation of NF-kappa B.
      Srikrishna, Geetha, et al.
      J. Immunol., 175: 5412-22 (2005)  2005

      Human16210648 16210648