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CB1036 Anti-S100A7 Mouse mAb (1A4)

This Anti-S100A7 Mouse mAb (1A4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of S100A7.

Anti-S100A7 Mouse mAb (1A4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-S100 Calcium Binding Protein A7

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CB1036
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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CB1036-100UG
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      Description
      OverviewRecognizes the ~10 kDa S100A7 protein in A431 cells.
      Catalogue NumberCB1036
      Brand Family Calbiochem®
      SynonymsAnti-S100 Calcium Binding Protein A7
      Application Data
      Detection of human S100A7 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human S100A7 by immunocytochemistry. Sample: A-431 cells fixed in paraformaldehyde. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlA-431 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human S100A7 (aa 1-102)
      Clone1A4
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CB1036-100UG 04055977228748

      Documentation

      Anti-S100A7 Mouse mAb (1A4) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2010 RFH
      SynonymsAnti-S100 Calcium Binding Protein A7
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human S100A7 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human S100A7 by immunocytochemistry. Sample: A-431 cells fixed in paraformaldehyde. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~10 kDa S100A7 protein.
      BackgroundS100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known.
      HostMouse
      ImmunogenFull-length, recombinant, human S100A7 (aa 1-102)
      Clone1A4
      IsotypeIgG₁
      Specieshuman
      Positive controlA-431 cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review