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MABF854 Anti-S100A9 Antibody, clone 6B4

This mouse monoclonal Anti-S100A9 Antibody, clone 6B4, Cat. No. MABF854 is validated for use in Western Blotting and ELISA, for the detection of S100A9.

Anti-S100A9 Antibody, clone 6B4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: S100A9, CAGB, CFAG, MRP14 UniProt Number: P06702
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MABF854
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF854
      DescriptionAnti-S100A9 Antibody, clone 6B4
      Alternate Names
      • Protein S100-A9
      • Calgranulin-B
      • Calprotectin L1H subunit
      • Leukocyte L1 complex heavy chain
      • Migration inhibitory factor-related protein 14
      • MRP-14
      • p14
      • S100 calcium-binding protein A9
      • S100A9
      Background InformationProtein S100-A9 (UniProt P06702; also known as Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, S100 calcium-binding protein A9, S100A9) is encoded by the S100A9 (also known as 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MRP14) gene (Gene ID 6280) in human. S100A9 is one of the 21 members of the S100 family of proteins. S100A8 (calgranulin-A), S100A9 (calgranulin-B) and the heterdimer S100A8/S100A9 (calprotectin) exert their immunological and inflammatory action via binding to TLR-4, RAGE and some other putative receptors. S100A8, calprotectin, and S100A9 are chemoattractants for neutrophils, and S100A9 is known to induce the expression and activation of beta2-integrins. S100A9 is also shown to stimulate neutrophil degranulation and phagocytosis, while both S100A8 and S100A9 can induce the secretion of inflammatory cytokines from human monocytes. In addition, S100A9 is shown to potentiate both fMLP- and GM-CSF-stimulated IL-8 production from neutrophils, although S100A9 on its own cannot induce neutrophil cytokine production in the absence of fMLP and GM-CSF.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-S100A9 Antibody, clone 6B4, Cat. No. MABF854 is validated for use in Western Blotting and ELISA, for the detection of S100A9.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected recombinant hS100A9 (rhS100A9), as well as endogenous hS100A9 in neutrophil extract and purified calprotectin (S100A8/S100A9 dimer), but not rhS100A8 & rhS100A12 or recombinant murine S100A8 & S100A9 (Dr Philippe Tessier, Laval University, Quebec, CA).
      ELISA Analysis: A representative lot detected recombinant human S100A9 (rhS100A9) by direct/non-sandwich ELISA, but not rhS100A8 & rhS100A12 or recombinant murine S100A8 & S100A9 (Dr Philippe Tessier, Laval University, Quebec, CA).
      ELISA Analysis: A representative lot detected recombinant human S100A9 (rhS100A9) as well as endogenous S100A9 in purified human calprotectin (S100A8/S100A9 dimer) by sandwich ELISA, but not rhS100A8 or rhS100A12 (Dr Philippe Tessier, Laval University, Quebec, CA).
      Biological Information
      ImmunogenRecombinant protein corresponding to human S100A9.
      Clone6B4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6B4 selectively detects human S100A9 with no cross-reactivity toward hS100A8, hS100A12, mS100A8, or mS100A9.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Not mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • S100A9
      • CAGB
      • CFAG
      • MRP14
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~13 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected S100A9 in 10 µg of human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF854 04055977177077

      Documentation

      Anti-S100A9 Antibody, clone 6B4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-S100A9 Antibody, clone 6B4 Certificates of Analysis

      TitleLot Number
      Anti-S100A9, clone 6B4 - 3321808 3321808
      Anti-S100A9, clone 6B4 - 3901767 3901767
      Anti-S100A9, clone 6B4 - 4288037 4288037
      Anti-S100A9, clone 6B4 - 4316731 4316731
      Anti-S100A9, clone 6B4 -Q2583298 Q2583298

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies