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ABF1060-25UG Anti-SARS-CoV-2 ACE2 binding domain

Anti-SARS-CoV-2 ACE2 binding domain, Cat. No. ABF1060, is a rabbit polyclonal antibody that detects Spike protein of SARS-CoV-2 virus and is tested for use in Immunofluorescence and Western Blotting.

ABF1060-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABF1060-25UG
      DescriptionAnti-SARS-CoV-2 ACE2 binding domain
      Alternate Names
      • SARS-CoV-2 Spike glycoprotein
      • S glycoprotein
      • E2
      • Peplomer protein
      Background InformationSARS-CoV-2 Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S gene (Gene ID: 43740568) in SARS-CoV-2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 protein, which is reported to be well conserved, is responsible for membrane fusion.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 5 mM sodium azide with 50% glycerol.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SARS-CoV-2 ACE2 binding domain, Cat. No. ABF1060, is a rabbit polyclonal antibody that detects Spike protein of SARS-CoV-2 virus and is tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesWestern Blotting Analysis: A 1:2,000 dilution from a representative lot detected SARS-CoV2 ACE2 binding domain in lysate from HEK293 cells transfected with cDNA encoding the SARS-CoV-2 binding domain) or GFP-SARS-CoV-2-binding domain fusion protein (Courtesy of Gerry Shaw Labs)

      Immunofluorescence Analysis: A 1:3,000 dilution from a representative lot detected SARS-CoV2 ACE2 binding domain in HEK293 cells transfected with cDNA encoding the SARS-CoV2 S-protein ACE2 binding domain (aa 308-541)(Courtesy of Gerry Shaw Labs)

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant fragment corresponding to the receptor binding domain (ACE2) of SARS-CoV-2 virus spike protein.
      EpitopeN-terminal half
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Spike protein of SARS-CoV-2 virus. It targets an epitope within the receptor binding domain (ACE2).
      IsotypeIgG1
      Species Reactivity
      • Virus
      Species Reactivity NotePredicted to react with SARS-CoV Virus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • S
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~165 kDa observed; 141.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant SARS-CoV-2 Spike protein (ECD).

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant SARS-CoV-2 Spike protein (ECD).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABF1060-25UG 04065265651516