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ST1713 Anti-SERPING1 Mouse mAb (3F4-1D9)

ST1713
  
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      Overview

      Replacement Information

      Key Spec Table

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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~80 kDa SERPING1 protein in human liver and kidney tissue.
      Catalogue NumberST1713
      Brand Family Calbiochem®
      SynonymsAnti-Serpin Peptide Inhibitor G1
      Application Data
      Detection of human SERPING1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-SERPING1 Mouse mAb (3F4-1D9) (Cat. No. ST1713) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SERPING1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human kidney tissue. Primary antibody: Anti-SERPING1 Mouse mAb (3F4-1D9) (Cat. No. ST1713) (1 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman liver tissue, Human kidney tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (1 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human SERPING1 (aa 1-501), expressed as a GST fusion protein
      Clone3F4-1D9
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Serpin Peptide Inhibitor G1
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (1 µg/ml)
      Application Data
      Detection of human SERPING1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-SERPING1 Mouse mAb (3F4-1D9) (Cat. No. ST1713) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SERPING1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human kidney tissue. Primary antibody: Anti-SERPING1 Mouse mAb (3F4-1D9) (Cat. No. ST1713) (1 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~80 kDa SERPING1 protein.
      BackgroundThis gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform.
      HostMouse
      ImmunogenFull-length, recombinant, human SERPING1 (aa 1-501), expressed as a GST fusion protein
      Clone3F4-1D9
      IsotypeIgG2a
      Specieshuman
      Positive controlHuman liver tissue, Human kidney tissue
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review