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ST1709 Anti-SKP1A Mouse mAb (1H8)

ST1709
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Overview

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Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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ST1709-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~18 kDa SKP1A protein in HeLa cells.
      Catalogue NumberST1709
      Brand Family Calbiochem®
      SynonymsAnti-S-Phase Kinase-Associated Protein 1
      Application Data
      Detection of human SKP1A by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SKP1A by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1-5 µg/ml
      Immunocytochemistry (10 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 53-161 human SKP1A, expressed as a GST fusion protein
      Clone1H8
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-SKP1A Mouse mAb (1H8) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-S-Phase Kinase-Associated Protein 1
      ApplicationImmunoblotting (1-5 µg/ml
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human SKP1A by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human SKP1A by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-SKP1A Mouse mAb (1H8) (Cat. No. ST1709) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~18 kDa SKP1A protein.
      BackgroundThis gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 53-161 human SKP1A, expressed as a GST fusion protein
      Clone1H8
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells
      Negative control293T cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review