Millipore Sigma Vibrant Logo

ABE993 Anti-SLUG (SNAI2)

Anti-SLUG (SNAI2), Cat. No. ABE993, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein SNAI2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-SLUG (SNAI2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SNAI2, SLUG, SLUGH UniProt Number: O43623
View Products on Sigmaaldrich.com
ABE993
100 μg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE993
      DescriptionAnti-SLUG (SNAI2)
      Alternate Names
      • Zinc finger protein SNAI2
      • Neural crest transcription factor Slug
      • Protein snail homolog 2
      Background InformationZinc finger protein SNAI2 (UniProt: O43623; also known as Neural crest transcription factor Slug, SLUG, Protein snail homolog 2) is encoded by the SNAI2 (also known as SLUG, SLUGH) gene (Gene ID: 6591) in human. SLUG is a transcriptional repressor that modulates both activator-dependent and basal transcription. It is expressed in most adult human tissues, but is not detected in peripheral blood leukocyte. It is observed in discrete foci in interphase nuclei and these nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting its association with active transcription or active splicing regions. SLUG is shown to repress BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. It contains a SNAG domain (aa 1-20) that resembles a histone H3-like structure and functions as a molecular hook for recruiting LSD1 to repress gene expression in metastasis. SLUG is shown to be involved in the generation and migration of neural crest cells. It also plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. In epidermal keratinocytes, it binds to the E-box in ITGA3 promoter and represses its transcription. SLUG is also involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. It can bind to E-box2 domain of BSG and activate its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. SLUG phosphorylation by GSK-3 beta results in cytoplasmic localization and degradation. Mutation in SNAI2 gene can cause Waardenburg syndrome 2D that is characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. (Ref.: Lin, Y et al. (2010). EMBO J. 29(11); 1803-16).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SLUG (SNAI2), Cat. No. ABE993, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein SNAI2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SLUG (SNAI2) in human bone marrow and human pancreas tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the N-terminal half of human Zinc finger protein SNAI2.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects SLUG/SNAI2 in human cells. It targets an epitope within 12 amino acids from the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Canine, Sheep, Rat, Bovine, and Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SNAI2
      • SLUG
      • SLUGH
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~31 kDa observed; 29.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected SLUG (SNAI2) in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE993 04054839410437