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DR1035 Anti-SMG1 Rabbit pAb

This Anti-SMG1 Rabbit pAb is validated for use in Immunoblotting for the detection of SMG1.

Anti-SMG1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ATM-Related Kinase, Anti-LIP, Anti-61E3.4, Anti-KIAA0421, Anti-ATX, Anti-PI-3-Kinase-Related Kinase SMG1

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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~340 kDa SMG protein in HeLa cell nuclear extracts.
Catalogue NumberDR1035
Brand Family Calbiochem®
SynonymsAnti-ATM-Related Kinase, Anti-LIP, Anti-61E3.4, Anti-KIAA0421, Anti-ATX, Anti-PI-3-Kinase-Related Kinase SMG1
Application Data
Detection of human SMG-1 by immunoblotting. Sample: Whole cell lysate (50 μg) from HeLa cells. Primary antibody: Anti-SMG-1 Rabbit pAb (Cat. No. DR1035) (0.1 μg/ml). Detection: chemiluminescence.
References
ReferencesAbraham, R.T., 2004. DNA Repair (Amst.) 3, 919.
Brumbaugh, K.M., et al. 2004. Mol. Cell. 14, 585.
Yamashita, A., et al. 2001. Genes Dev. 15, 2215.
Product Information
FormLiquid
FormulationIn 100 mM NaCl, 10 mM phosphate, pH 7.2.
Positive controlHeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (0.1 µg/ml)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 1625-167 of human SMG1
ImmunogenHuman
Epitopewithin amino acids 1625-1675
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1035 0

Documentation

Anti-SMG1 Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-SMG1 Rabbit pAb Certificates of Analysis

TitleLot Number
DR1035

References

Reference overview
Abraham, R.T., 2004. DNA Repair (Amst.) 3, 919.
Brumbaugh, K.M., et al. 2004. Mol. Cell. 14, 585.
Yamashita, A., et al. 2001. Genes Dev. 15, 2215.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-June-2013 JSW
SynonymsAnti-ATM-Related Kinase, Anti-LIP, Anti-61E3.4, Anti-KIAA0421, Anti-ATX, Anti-PI-3-Kinase-Related Kinase SMG1
ApplicationImmunoblotting (0.1 µg/ml)
Application Data
Detection of human SMG-1 by immunoblotting. Sample: Whole cell lysate (50 μg) from HeLa cells. Primary antibody: Anti-SMG-1 Rabbit pAb (Cat. No. DR1035) (0.1 μg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~340 kDa SMG protein.
BackgroundSMG-1 (ACC# NP 055907), a PIKK family member, was named based on its homology to the Caenorhabditis elegans protein CeSMG-1. SMG-1 functions in a RNA surveillance pathway termed nonsense-mediated mRNA decay (NMD). NMD mediates the rapid degradation of mRNAs bearing premature termination codons (PTCs), which threaten cell survival by producing truncated, potentially dominant-interfering proteins. Inhibition of SMG-1 activity in mammalian cells leads to suppression of NMD. SMG-1 regulates NMD in part through the phosphorylation of hUpf1, a conserved component of the NMD machinery in metazoan cells. SMG-1 phosphorylates at least four sites in the carboxy-terminal, S/T-Q-rich domain of hUpf1, and mutational analysis indicated that these phosphorylation events are important for NMD activity. SMG-1 has also been called ATX to acknowledge its similarity to the genome surveillance kinases ATM and ATR. In addition to its critical role in NMD, SMG-1 is centrally involved in the regulation of the genotoxic stress response machinery in mammalian cells. Cellular exposure to ultraviolet (UV) light or IR stimulates SMG-1 kinase activity and SMG-1 function is required for maximal p53 phosphorylation and stabilization in radiation-damaged cells. Moreover, SMG-1-deficient cells are sensitized to IR-induced stress.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 1625-167 of human SMG1
Epitopewithin amino acids 1625-1675
IsotypeIgG
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationIn 100 mM NaCl, 10 mM phosphate, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesAbraham, R.T., 2004. DNA Repair (Amst.) 3, 919.
Brumbaugh, K.M., et al. 2004. Mol. Cell. 14, 585.
Yamashita, A., et al. 2001. Genes Dev. 15, 2215.