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MABD390 Anti-SPRY1 Antibody, clone 7E6.2

This mouse monoclonal Anti-SPRY1 Antibody, clone 7E6.2, Cat. No. MABD390 is validated for use in Western Blotting for the detection of SPRY1.

Anti-SPRY1 Antibody, clone 7E6.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Gene Symbol: SPRY1 UniProt Number: O43609
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MABD390
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABD390
      DescriptionAnti-SPRY1 Antibody, clone 7E6.2
      Alternate Names
      • Protein sprouty homolog 1
      • Spry-1
      Background InformationProtein sprouty homolog 1 (UniProt O43609; also known as Spry-1) is encoded by the SPRY1 gene (Gene ID 10252) in human. Sprouty was originally identified in Drosophila as a negative regulator of fibroblast growth factor (FGF) signaling during tracheal development. There exist four mammalian homologs (SPRY1-4) that likewise mediate feedback inhibition against growth factor-/receptor tyrosine kinase- (RTK-) dependent Ras/mitogen-activated protein (MAP) kinase signaling pathway. Loss of function studies in mice show that SPRY1 and SPRY2 play critical roles during early development of multiple organ rudiments, including external genitalia, eye, kidney, inner ear, tooth, and cortical patterning in the brain. In addition, evidences indicate that SPRY1 plays an antiproliferative and tumor-suppressing role in cancers. SPRY1 downregulation is reported in various cancer types, including prostate, breast, and ovarian cancers, and SPRY1 expresion has been proposed as a marker of good prognosis in patients with renal cell carcinoma.
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse IgG1 hybridoma culture supernatant without azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-SPRY1 Antibody, clone 7E6.2, Cat. No. MABD390 is validated for use in Western Blotting for the detection of SPRY1.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SPRY1 in 10 µg of SK-MEL-2 human skin melanoma cell lysate.
      Biological Information
      ImmunogenGST-tagged recombinant human SPRY1 N-terminal fragment.
      Clone7E6.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7E6.2 detects the ~35 kDa human SPRY1 by targeting an N-terminal region epitope.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SPRY1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~35 kDa observed. 35.12 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.


      Western Blotting Analysis: A 1:1,000 dilution of this hybridoma culture supernatant detected SPRY1 in 10 µg of HepG2 human hepatocellular carcinoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABD390 04054839086205