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MABS1997-100UG Anti-ST8SIA1 Antibody, clone HIC0-3C5

Anti-ST8SIA1, clone HIC0-3C5, Cat. No. MABS1997, is a mouse monoclonal antibody that detects Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase and has been tested for use in Flow Cytometry, Immunoflurescence, and Immunohistochemistry.

Anti-ST8SIA1 Antibody, clone HIC0-3C5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: ST8SIA1, SIAT8, SIAT8A UniProt Number: Q92185
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MABS1997-100UG
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IF, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1997-100UG
      DescriptionAnti-ST8SIA1 Antibody, clone HIC0-3C5
      Alternate Names
      • Alpha-N-acetylneuraminide alpha-2
      • 8-sialyltransferase
      • EC: 2.4.99.8
      • Alpha-2
      • 8-sialyltransferase 8A
      • Ganglioside GD3 synthase
      • Ganglioside GT3 synthase
      • Sialyltransferase 8A
      • SIAT8-A
      • Sialyltransferase St8Sia I
      • ST8SiaI
      Background InformationAlpha-N-acetylneuraminide alpha-2,8-sialyltransferase (UniProt: Q92185; also known as EC: 2.4.99.8, Alpha-2,8-sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, Sialyltransferase 8A, SIAT8-A, Sialyltransferase St8Sia I, ST8SiaI) I encoded by the ST8SIA1 (also known as SIAT8, SIAT8A) gene (Gene ID: 6489) in human. SIAT8-A is a single-pass type II membrane protein that is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. It is involved in the production of gangliosides GD3 and GT3 from GM3. It catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3 in a successive manner. It is also involved in the pathway protein glycosylation. SIAT8-A contains a cytoplasmic domain (aa 1-29); a transmembrane domain (aa 30-48); and a lumenal domain (aa 49-356). Two isoforms of SIAT8-A have been described that are produced by alternative splicing. (Ref.: Dorrell, C., et al. (2016). Nat. Commun. 7; 11756).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-ST8SIA1, clone HIC0-3C5, Cat. No. MABS1997, is a mouse monoclonal antibody that detects Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase and has been tested for use in Flow Cytometry, Immunoflurescence, and Immunohistochemistry.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesFlow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).

      Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
      Biological Information
      ImmunogenTrypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.
      CloneHIC0-3C5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).
      IsotypeIgMκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ST8SIA1
      • SIAT8
      • SIAT8A
      UniProt Number
      Molecular Weight40.52 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human pancreatic tissue sections.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected ST8SIA1 in human pancreatic tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1997-100UG 04054839540172