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MABT892 Anti-SUN1 Antibody, clone 12.10F

This mouse monoclonal Anti-SUN1 Antibody, clone 12.10F, Cat. No. MABT892 is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN1.

Anti-SUN1 Antibody, clone 12.10F MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Sun1, Unc84a UniProt Number: Q9D666
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MABT892
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT892
      DescriptionAnti-SUN1 Antibody, clone 12.10F
      Alternate Names
      • SUN domain-containing protein 1
      • Protein unc-84 homolog A
      • Sad1/unc-84 protein-like 1
      Background InformationSUN domain-containing protein 1 (UniProt Q9D666; also known as Protein unc-84 homolog A, Sad1/unc-84 protein-like 1) is encoded by the Sun1 (also known as Unc84a) gene (Gene ID 77053) in murine species. SUN1 is a component of the SUN-protein-containing complexes known as LINC (linker of nucleoskeleton and cytoskeleton), which link the nucleoskeleton and cytoskeleton structures together in a scaffold like fashion. SUN1 is required for interkinetic nuclear migration and nucleokinesis as well as nucleus-centrosome coupling during radial neuronal migration and glial migration in the cortex. SUN1 also is important during meiosis and is required for proper gametogenesis. It also participates in anchoring chromosome movement in the prophase of meiosis and in selective gene expression of coding and non-coding RNAs required for gametogenesis. Additionally, it has a vital role in the distribution of nuclear pore complexes on the nuclear membrane. Interestingly, LINC complexes are also critical for vital functions such as hearing.
      References
      Product Information
      FormatPurified
      PresentationPurified mous IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-SUN1 Antibody, clone 12.10F, Cat. No. MABT892 is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN1.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected SUN1 nuclear immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized C2C12 murine myoblasts.

      Immunocytochemistry Analysis: A representative lot immunostained nucleus of C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore).

      Western Blotting Analysis: A representative lot detected greatly reduced SUN1 isoforms target bands in lysate from SUN1 siRNA-treated C2C12 murine myoblasts (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR, Singapore).
      Biological Information
      ImmunogenGST-tagged recombinant fusion protein composed of murine SUN1 regions encoded by exons 6, 8, and 10.
      Clone12.10F
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 12.10F was raised against a fusion construct composed of murine SUN1 exon 6-, 8-, and 10-encoded regions that are present in murine spliced isoforms 1, 3, and 4 reported by UniProt (Q9D666). The epitope location among these three regions has not been mapped and reactivity toward murine spliced isoform 3 is expected only if the epitope resides within exon 1- or 4-encoded region.
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat. Not Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Sun1
      • Unc84a
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~102 kDa oberved. 102.0/88.20/97.92/94.51 kDa (mouse isoform 1/2/3/4) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in C2C12 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN1 in 10 µg of C2C12 murine myoblast lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT892 04054839108723