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MABN70 Anti-Slo1 Antibody, clone L6/60

Detect Slo1 using this Anti-Slo1 Antibody, clone L6/60 validated for use in IH, WB & IC.

Anti-Slo1 Antibody, clone L6/60 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: SLO1, KCa1.1, SAKCA, SLO, k(VCA)alpha, mSLO1, BKTM, MaxiK, KCNMA1, hSlo, KCNMA3, Slo1 UniProt Number: Q08460
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MABN70
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MIHC, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN70
      DescriptionAnti-Slo1 Antibody, clone L6/60
      Alternate Names
      • potassium large conductance calcium-activated channel, subfamily M, alpha member 1
      • Slo homolog
      • stretch-activated Kca channel
      • Maxi K channel
      • Drosophila slowpoke-like
      • Calcium-activated potassium channel, subfamily M subunit alpha-1
      • Slowpoke homolog
      • BK channel
      • calcium-activated potassium channel subunit alpha-1
      • BKCA alpha
      • BKCA alpha subunit
      • K(VCA)alpha
      • SLO-ALPHA
      • Slo-alpha
      Background InformationSlo1 (Calcium-activated potassium channel subunit alpha-1) may not only be activated by membrane depolarization but may also be by an increase in cytosolic Ca2+. The concentration of cytosolic Mg2+ also activates this potassium channel. Found to play a part in the repolarization of the membrane potential due to the fact that it diminishes the excitatory voltage in the cell.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain membrane tissue lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Slo1 using this Anti-Slo1 Antibody, clone L6/60 validated for use in IH, WB & IC.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: 1:500 dilution from a previous lot detected Slo1 in rat cerebellum tissue.

      Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Misonou, H., et al. (2006). J Comp Neurol. 496(3):289–302.)
      Biological Information
      ImmunogenRecombinant protein corresponding to the S9-S10 segments of mouse Slo1.
      EpitopeS9-S10 segments
      CloneL6/60
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes S9-S10 segments of Slo1.
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with rat. Predicted to react with mouse based on immunogen design.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLO1
      • KCa1.1
      • SAKCA
      • SLO
      • k(VCA)alpha
      • mSLO1
      • BKTM
      • MaxiK
      • KCNMA1
      • hSlo
      • KCNMA3
      • Slo1
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca2+ that mediates export of K+. It is also activated by the concentration of cytosolic Mg2+. Its activation dampens the excitatory events that elevate the cytosolic Ca2+ concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca2+, caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).

      ENZYME REGULATION: Ethanol and carbon monoxide-bound heme increase channel activation. Heme inhibits channel activation (By similarity).

      SUBUNIT STRUCTURE: Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Beta subunits are accessory, and modulate its activity.

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (By similarity).

      DOMAIN: The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4.

      The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor.

      The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium.

      The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca2+ and Mg2+.

      The calcium bowl constitutes one of the Ca2+ sensors and probably acts as a Ca2+-binding site. There are however other Ca2+ sensors regions required for activation of the channel.

      The heme-binding motif mediates inhibition of channel activation by heme. Carbon monoxide-bound heme leads to increased channel activation (By similarity).

      PTM: Phosphorylated Probable. Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.

      MISCELLANEOUS: The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel contains binding sites for Ca2+ and Mg2+.

      SEQUENCE SIMILARITIES: Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa1.1/KCNMA1 sub-subfamily. [View classification]

      Contains 1 RCK N-terminal domain.

      SEQUENCE CAUTION: The sequence AAA50215.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~100 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain membrane tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Slo1 on 10 µg of rat brain membrane tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN70 04053252414244

      Documentation

      Anti-Slo1 Antibody, clone L6/60 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Slo1 Antibody, clone L6/60 Certificates of Analysis

      TitleLot Number
      Anti-Slo1, clone L6/60 - 2360030 2360030
      Anti-Slo1, clone L6/60 - 2424141 2424141
      Anti-Slo1, clone L6/60 - 2438272 2438272
      Anti-Slo1, clone L6/60 - 2266758 2266758
      Anti-Slo1, clone L6/60 - 3560682 3560682
      Anti-Slo1, clone L6/60 - 3990661 3990661
      Anti-Slo1, clone L6/60 - 4043517 4043517
      Anti-Slo1, clone L6/60 - 4156831 4156831
      Anti-Slo1, clone L6/60 - 4301967 4301967
      Anti-Slo1, clone L6/60 - NRG1818968 NRG1818968
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