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567365 Anti-Smac/DIABLO (222-237) Rabbit pAb

567365
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      OverviewRecognizes the ~23 kDa mature form of Smac/DIABLO.

      This product has been discontinued.





      Catalogue Number567365
      Brand Family Calbiochem®
      Application Data
      Detection of human Smac/DIABLO by immunoblotting. Sample: Tissue lysate from human heart without blocking peptide (lane A) or with blocking peptide (lane B). Primary antibody: Anti-Smac/DIABLO (222-237) Rabbit pAb (Cat. No. 567365) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesDu, C., et al. 2000. Cell 102, 33.
      Chai, J., et al. 2000. Nature 406, 855.
      Liu, Z., et al. 2000. Nature 408, 1004.
      Verhagen, A.M., et al. 2000. Cell 102, 43.
      Wu, G., et al. 2000. Nature 408, 1008.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (0.5-1 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide (SDEGADQEEEAYLRED) corresponding to amino acids 222-237 of mouse Smac/DIABLO
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Smac/DIABLO (222-237) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Smac/DIABLO (222-237) Rabbit pAb Certificates of Analysis

      TitleLot Number
      567365

      References

      Reference overview
      Du, C., et al. 2000. Cell 102, 33.
      Chai, J., et al. 2000. Nature 406, 855.
      Liu, Z., et al. 2000. Nature 408, 1004.
      Verhagen, A.M., et al. 2000. Cell 102, 43.
      Wu, G., et al. 2000. Nature 408, 1008.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-August-2007 RFH
      ApplicationImmunoblotting (0.5-1 µg/ml)
      Application Data
      Detection of human Smac/DIABLO by immunoblotting. Sample: Tissue lysate from human heart without blocking peptide (lane A) or with blocking peptide (lane B). Primary antibody: Anti-Smac/DIABLO (222-237) Rabbit pAb (Cat. No. 567365) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~23 kDa mature form of Smac/DIABLO.
      BackgroundSmac, also known as DIABLO, is a mitochondrial protein that promotes cytochrome c-dependent caspase activation by neutralizing inhibitor of apoptosis proteins (IAPs). Smac functions at the levels of both the Apaf-1-caspase-9 apoptosome and effector caspases. Smac is translocated to the cytosol during apoptosis. Mitochondrial import and cleavage of its signal peptide are required for Smac to gain its apoptotic activity. Processing of Smac to the mature form is independent of apoptosis.
      HostRabbit
      Immunogen speciesMouse
      Immunogena synthetic peptide (SDEGADQEEEAYLRED) corresponding to amino acids 222-237 of mouse Smac/DIABLO
      IsotypeIgG
      Specieshuman, mouse, rat
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDu, C., et al. 2000. Cell 102, 33.
      Chai, J., et al. 2000. Nature 406, 855.
      Liu, Z., et al. 2000. Nature 408, 1004.
      Verhagen, A.M., et al. 2000. Cell 102, 43.
      Wu, G., et al. 2000. Nature 408, 1008.