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ABE1975 Anti-Srg3/BAF155 Antibody

Anti-Srg3/BAF155 Antibody is an antibody against Srg3/BAF155 for use in Western Blotting, Chromatin Immunoprecipitation (ChIP).

Anti-Srg3/BAF155 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: Smarcc1, Baf155, Srg3 UniProt Number: P97496
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ABE1975
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE1975
      DescriptionAnti-Srg3/BAF155 Antibody
      Alternate Names
      • SWI/SNF complex subunit SMARCC1
      • BAF155
      • BRG1-associated factor 155
      • SWI/SNF complex 155 kDa subunit
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1
      • SWI3-related protein
      Background InformationSWI/SNF complex subunit SMARCC1 (UniProt P97496; also known as BAF155, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SWI3-related protein) is encoded by the Smarcc1 (also known as Baf155, Srg3) gene (Gene ID 20588) in murine species. SMARCC1/BAF155/SRG3 is a core component of the SWI/SNF complex that coordinates the expression of specific genes crucial for germinal center (GC) formation, including Blimp-1 and Bcl-6. The SWI/SNF is a chromatin-remodeling complex that drives conformational changes of nucleosomes in an ATP-dependent manner. SWI/SNF complex is required for the differentiation of GC B and T follicular helper (Tfh) cells. Smarcc1/Baf155/Srg3-knockout mice showed impaired differentiation of GC B and Tth cells in response to T cell-dependent antigen challenge. The SWI/SNF complex consists of at least 12 different subunits, including BRG1 with ATPase activity and SMARCC1/BAF155/SRG3 that controls the complex stability. SMARCC1/BAF155/SRG3 contains an N-terminal acidic region (23% Asp/Glu content from a.a. 211-441), a DNA-binding SWIRM domain (a.a. 448-545), a histone-interacting SANT domain (a.a. 617-668), a leucine-zipper motif (a.a. 899-942), and a C-terminal glutamine- and proline-rich region (a.a. 768-862 & 977-1104).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Srg3/BAF155 Antibody is an antibody against Srg3/BAF155 for use in Western Blotting, Chromatin Immunoprecipitation (ChIP).
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: A representative lot detected Srg3 immunoprecipitated from the nuclear extracts of human Raji and murine BAL17 B cells (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
      Western Blotting Analysis: A representative lot detected the ~155 kDa target Srg3 band and a ~170 kDa cross-reactive band in murine thymus and lymph node lysates. The ~170 kDa band likely represents the murine ortholog of human BAF170 protein (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836).
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Srg3 occupancy at the proximal promoter and BRE (Bcl-6 responsive element) regions of the Blimp-1 locus in germinal center (GC) B cells, but not in plasma B cells (PCs) (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Srg3 occupacy at the Blimp-1 locus using chromatin preprations from untreated murine BAL17 B lymphoma cells, but not Brg1/Srg3-depleted BAL17 cells following siRNAs treatment (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Srg3 and its associated proteins from the nuclear extracts of human Raji and murine BAL17 B cells (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
      Immunoprecipitation Analysis: A representative lot co-immunoprecipitated a ~195 kDa SWI2-like protein with the ~155 kDa Srg3 protein from murine thymus cell extracts (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836).
      Biological Information
      ImmunogenGST-tagged recombinant mouse Srg3/BAF155 C-terminal fragment.
      EpitopeC-terminal region.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityReacts with both mouse and human Srg3/BAF155 proteins. Reproted to exhibit cross-reactivity toward a ~170 kDa murine ortholog of human BAF170 protein (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836). Expected to react with also murine Srg3/Smarcc1 spliced isoform 2 reported by UniProt (UniProt P97496-2).
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Smarcc1
      • Baf155
      • Srg3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~155 kDa observed. 122.9 kDa (human and mouse SRG3) and 120.1 kDa (mouse spliced isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Srg3/BAF155 in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1975 04055977335231