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MAB5258-I Anti-Synaptophysin Antibody, clone SY38

Anti-Synaptophysin, clone SY38, Cat. No. MAB5258-I, is a highly specific mouse monoclonal antibody that targets Synaptophysin and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-Synaptophysin Antibody, clone SY38 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: SYP UniProt Number: P20488
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MAB5258-I
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, B, H, RIH(P), WB, IF, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5258-I
      DescriptionAnti-Synaptophysin Antibody, clone SY38
      Alternate Names
      • Synaptophysin
      • Major synaptic vesicle protein p38
      Background InformationSynaptophysin (UniProt P20488; also known as Major synaptic vesicle protein p38) is encoded by the SYP gene (Gene ID 280937) in bovine species. Synaptophysin is a 38 kDa four-transmembrane glycoprotein on synaptic vesicle (SV) with both its N- and C-termini exposed cytoplasmically. Synaptophysin forms homomultimers in the SV membrane and is heavily phosphorylated on tyrosine residues in its C-terminal pentapeptide repeats. Synapthophysin is also known to interact with SV v-SNARE protein synaptobrevin II (sybII) when sybII is not engaged with other SNARE proteins, suggesting a role of synaptophysin in sybII retrieval during SV endocytosis. Consistently, synaptophysin-knockout neurons display reduced level of sybII at their nerve terminals.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Synaptophysin, clone SY38, Cat. No. MAB5258-I, is a highly specific mouse monoclonal antibody that targets Synaptophysin and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptophysin in human cerebellum and cerebral cortex tissue sections.

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Synaptophysin in 10 µg of rat hippocampus and human whole brain tissue lysates.

      Immunocytochemistry Analysis: Representative lots immunostained presynaptic vesicles of 4% formaldehyde-fxied, 0.2% Triton X-100-permeabilzied primary mouse hippocampal neurons by fluorescent immunocytochemistry (Hu, X., et al. (2008). J. Neurosci. 28(49):13094-13105; Tarr, P.T., and Edwards, P.A. (2008). J. Lipid Res. 49(1):169-182).

      Immunofluorescence Analysis: A representative lot immunostained presynaptic membrane of neurons by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized, OCT-embedded rat spinal cord cryosections (Stück, E.D., et al. (2012). Neural Plast. 2012:261345).

      Immunofluorescence Analysis: A representative lot immunostained synaptophysin around a large neuron in the ventral horn of the lumbar (L3/L4) segment by fluorescent immunohistochemistry staining of 2% paraformaldehyde/0.2% parabenzoquinone-fixed free-floating rat spinal cord sections (Macias, M., et al. (2009). BMC Neurosci. 10:144).

      Immunofluorescence Analysis: A representative lot immunostained synaptophysin in bovine (pancreas) and human (pancreas, pheochromocytoma, and islet-cell carcinoma) frozen tissue sections (Wiedenmann, B., et al. (1986). Proc. Natl. Acad. Sci. U.S.A. 83(10):3500-3504).

      Western Blotting Analysis: A representative lot detected an upregulated synaptophysin expression in human iPSCs with a 4-bp deletion in DISC1 gene (Wen, Z., et al. (2014). Nature. 515(7527):414-418).

      Western Blotting Analysis: A representative lot detected synaptophysin distribution among PC12 rat pheochromocytoma cell membrane fractions (Salazar, G., et al. (2005). Mol. Biol. Cell. 16(8):3692-3704).

      Western Blotting Analysis: A representative lot detected only synaptophysin recombinant contructs that contained the flexible segment -SGGGG- in the center of the c-terminal pentapeptide repeats (Knaus, P., and Betz, H. (1990). FEBS Lett. 261(2):358-360).
      Biological Information
      ImmunogenPresynaptic vesicles from bovine brain (Wiedenmann, B., and Franke, W.W. (1985). Cell. 41(3):1017-1028).
      CloneSY38
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone SY38 detects synaptophysin and stains synaptophysin-containing vesicles by targeting the flexible segment -SGGGG- in the center of the C-termimal pentapeptide repeats (Knaus, P., and Betz, H. (1990). FEBS Lett. 261(2):358-360).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Bovine
      • Human
      • Rat
      Species Reactivity NoteBovine, Human, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SYP
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~38 kDa observed. 33.91/34.03/33.31 kDa (bovine/mouse/rat), 33.85/20.76 kDa (human isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse hypothalamus tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Synaptophysin in 10 µg of mouse hypothalamus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5258-I 04054839092077

      Documentation

      Anti-Synaptophysin Antibody, clone SY38 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Synaptophysin Antibody, clone SY38 Certificates of Analysis

      TitleLot Number
      Anti-Synaptophysin, clone SY38 - 3226282 3226282
      Anti-Synaptophysin, clone SY38 - 3314244 3314244
      Anti-Synaptophysin, clone SY38 - 3475628 3475628
      Anti-Synaptophysin, clone SY38 - 3554706 3554706
      Anti-Synaptophysin, clone SY38 - 3861334 3861334
      Anti-Synaptophysin, clone SY38 - 3937800 3937800
      Anti-Synaptophysin, clone SY38 - 4095086 4095086
      Anti-Synaptophysin, clone SY38 - 4151746 4151746
      Anti-Synaptophysin, clone SY38 - 4263830 4263830
      Anti-Synaptophysin, clone SY38 -2814664 2814664
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