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PC491 Anti-TACE (807 - 823) Rabbit pAb

This Anti-TACE (807 - 823) Rabbit pAb is validated for use in Immunoblotting for the detection of TACE (807 - 823).

Anti-TACE (807 - 823) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TNF-α Converting Enzyme, Anti-ADAM17

PC491
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.





Recognizes the ~130 kDa TACE protein in HeLa and Jurkat cells.
Catalogue NumberPC491
Brand Family Calbiochem®
SynonymsAnti-TNF-α Converting Enzyme, Anti-ADAM17
Application Data
Detection of human TACE by immunoblotting. Samples: Whole cell lysate from HeLa cells (lanes 1,3) or Jurkat cells (lanes 2,4). Primary antibody: Anti-TACE (807 - 823) Rabbit pAb (Cat. No. PC491) pre-incubated without blocking peptide (lanes 1,2) or with blocking peptide (lanes 3,4) (1:500). Detection: chemiluminescence.
References
ReferencesMizul, Y., et al. 1999. Gene. 233, 67.
Buxbaum, J.D., et al. 1998. J. Biol. Chem. 273, 27765.
Giegel, D.A. and Cheplinm, D.D. 1998. Agents Actions Suppl. 491, 1.
Peschon, J.J., et al. 1998. Science 282, 1281.
Black, R.A., et al. 1997. Nature 385, 729.
Moss, M.L., et al. 1997. Nature 385, 733.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlHeLa or Jurkat cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:500-1:2000)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (ASFKLQRQNRVDSKETE) corresponding to amino acids 807-823 of human TACE
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC491 0

Documentation

Anti-TACE (807 - 823) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-TACE (807 - 823) Rabbit pAb Certificates of Analysis

TitleLot Number
PC491

References

Reference overview
Mizul, Y., et al. 1999. Gene. 233, 67.
Buxbaum, J.D., et al. 1998. J. Biol. Chem. 273, 27765.
Giegel, D.A. and Cheplinm, D.D. 1998. Agents Actions Suppl. 491, 1.
Peschon, J.J., et al. 1998. Science 282, 1281.
Black, R.A., et al. 1997. Nature 385, 729.
Moss, M.L., et al. 1997. Nature 385, 733.

Citations

Title
  • Daniel W. Lambert, et al. (2005) Tumor Necrosis Factor- Convertase (ADAM17) Mediates Regulated Ectodomain Shedding of the Severe-acute Respiratory Syndrome-Coronavirus (SARS-CoV) Receptor, Angiotensin-converting Enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision11-October-2007 RFH
    SynonymsAnti-TNF-α Converting Enzyme, Anti-ADAM17
    ApplicationImmunoblotting (1:500-1:2000)
    Application Data
    Detection of human TACE by immunoblotting. Samples: Whole cell lysate from HeLa cells (lanes 1,3) or Jurkat cells (lanes 2,4). Primary antibody: Anti-TACE (807 - 823) Rabbit pAb (Cat. No. PC491) pre-incubated without blocking peptide (lanes 1,2) or with blocking peptide (lanes 3,4) (1:500). Detection: chemiluminescence.
    DescriptionRabbit polyclonal antibody purified by ion exchange chromatography. Recognizes the TACE protein.
    BackgroundTumor-necrosis factor-α is a proinflammatory cytokine that contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE, a ~130 kDa protein, is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFα, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (ASFKLQRQNRVDSKETE) corresponding to amino acids 807-823 of human TACE
    IsotypeIgG
    Specieshuman, mouse, rat
    Positive controlHeLa or Jurkat cells
    FormLiquid
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAntibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesMizul, Y., et al. 1999. Gene. 233, 67.
    Buxbaum, J.D., et al. 1998. J. Biol. Chem. 273, 27765.
    Giegel, D.A. and Cheplinm, D.D. 1998. Agents Actions Suppl. 491, 1.
    Peschon, J.J., et al. 1998. Science 282, 1281.
    Black, R.A., et al. 1997. Nature 385, 729.
    Moss, M.L., et al. 1997. Nature 385, 733.
    Citation
  • Daniel W. Lambert, et al. (2005) Tumor Necrosis Factor- Convertase (ADAM17) Mediates Regulated Ectodomain Shedding of the Severe-acute Respiratory Syndrome-Coronavirus (SARS-CoV) Receptor, Angiotensin-converting Enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
    		•