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AP1168 Anti-TNFSF14 Mouse mAb (4E3)

This Anti-TNFSF14 Mouse mAb (4E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of TNFSF14.

Anti-TNFSF14 Mouse mAb (4E3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tumor Necrosis Factor Superfamily Member 14, Anti-Tumor Necrosis Factor Receptor Like 2

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AP1168
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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AP1168-100UG
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      Description
      OverviewRecognizes the TNFSF14 protein.
      Catalogue NumberAP1168
      Brand Family Calbiochem®
      SynonymsAnti-Tumor Necrosis Factor Superfamily Member 14, Anti-Tumor Necrosis Factor Receptor Like 2
      Application Data
      Detection of human TNFSF14 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TNFSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman spleen tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 61-170 human TNFSF14, expressed as a GST fusion protein
      Clone4E3
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1168-100UG 04055977212693

      Documentation

      Anti-TNFSF14 Mouse mAb (4E3) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-October-2010 JLN
      SynonymsAnti-Tumor Necrosis Factor Superfamily Member 14, Anti-Tumor Necrosis Factor Receptor Like 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human TNFSF14 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TNFSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the TNFSF14 protein.
      BackgroundTNFSF14 is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, a member of the tumor necrosis factor receptor superfamily, whereby TNFRSF14 is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 61-170 human TNFSF14, expressed as a GST fusion protein
      Clone4E3
      IsotypeIgG2a
      Specieshuman
      Positive controlHuman spleen tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review