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AP1163 Anti-TP53BP2 Mouse mAb (3F8)

This Anti-TP53BP2 Mouse mAb (3F8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of TP53BP2.

Anti-TP53BP2 Mouse mAb (3F8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tumor Protein p53 Binding Protein 2

AP1163
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~120 kDa TP53BP2 protein in HepG2 cells and human testes tissue.
      Catalogue NumberAP1163
      Brand Family Calbiochem®
      SynonymsAnti-Tumor Protein p53 Binding Protein 2
      Application Data
      Detection of human TP53BP2 by iummunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-TP53BP2 Mouse mAb (3F8) (Cat. No. AP1163) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TP53BP2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-TP53BP2 Mouse mAb (3F8) (Cat. No. AP1163) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHepG2 cells, Human testes tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 511-610 human TP53BP2, expressed as a GST fusion protein
      Clone3F8
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1163 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-Tumor Protein p53 Binding Protein 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human TP53BP2 by iummunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-TP53BP2 Mouse mAb (3F8) (Cat. No. AP1163) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human TP53BP2 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-TP53BP2 Mouse mAb (3F8) (Cat. No. AP1163) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~120 kDa TP53BP2 protein.
      BackgroundTP53BP2 is a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. It is localized to the perinuclear region of the cytoplasm, and regulates apoptosis and cell growth through interactions with other regulatory molecules including members of the p53 family.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 511-610 human TP53BP2, expressed as a GST fusion protein
      Clone3F8
      IsotypeIgG2a
      Specieshuman
      Positive controlHepG2 cells, Human testes tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review