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MABN769 Anti-TRPM2 Antibody, clone 9D8.1

Anti-TRPM2 Antibody, clone 9D8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: TRPM2, EREG1, KNP3, LTRPC2, TRPC7, NUDT9H, NUDT9L1 UniProt Number: O94759
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MABN769
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWBMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMABN769
      DescriptionAnti-TRPM2 Antibody, clone 9D8.1
      Alternate Names
      • Transient receptor potential cation channel subfamily M member 2
      • Estrogen-responsive element-associated gene 1 protein
      • Long transient receptor potential channel 2
      • LTrpC-2
      • LTrpC2
      • Transient receptor potential channel 7
      • TrpC7
      Background InformationTransient receptor potential cation channel subfamily M member 2 (EC 3.6.1.13; UniProt O94759; also known as Estrogen-responsive element-associated gene 1 protein, Long transient receptor potential channel 2, LTrpC-2, LTrpC2, Transient receptor potential channel 7, TrpC7) is encoded by the TRPM2 (also known as EREG1, KNP3, LTRPC2, TRPC7, NUDT9H, NUDT9L1) gene (Gene ID 7226) in human. The human TRPM2 is an 1,503-amino acid, six-transmembrane protein with both N- and C-termini exposed intracellularly. TRPM2 contains a pore-forming loop domain located between the fifth and sixth transmembrane region. The N-terminal cytomplasmic region of TRPM2 contains four homologous domains with unknown function and a calmodulin-/CaM-binding IQ-like motif, which is important for channel activation. Its C-terminal cytomplasmic region contains the TRP box, a coiled-coil domain required for the assembly of TRPM2 channels, and an adenosine diphosphate ribose (ADPR) pyrophosphatase domain (Nudix-like domain or NUDT9 homology domain [NUDT9-H]). ADPR binds to the NUDT9-H domain and, in the presence of Ca2+, gates channel opening. TRPM2 activation also results in hydrolysis of ADPR into adenine monophosphate (AMP) and ribose-5-phosphate. TRPM2 is a temperature-sensitive Ca2+-permeable ion channel expressed in various tissues, including brain, lungs, liver, spleen, heart, salivary gland, and pancreas. TRPM2 is involved in redox response, cell proliferation, and insulin secretion by pancreatic β cells. TRPM2 plays a pivotal role in the death of CA1 pyramidal cells and in the development of post-ischemic brain injury. TRPM2 defect is associated with central nervous system disorders such as bipolar disorder type I, amyotrophic lateral sclerosis, Parkinson’s disease, and dementia.
      References
      Product Information
      FormatCulture Supernatant
      PresentationMouse monoclonal IgG2aκ in supernatant without preservatives.
      Quality LevelMQ100
      Applications
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminal cytoplasmic domain of human TRPM2.
      EpitopeN-terminal cytoplasmic domain.
      Clone9D8.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all three spliced isoforms reported by UniProt (O94759).
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRPM2
      • EREG1
      • KNP3
      • LTRPC2
      • TRPC7
      • NUDT9H
      • NUDT9L1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~170 kDa observed. 171.2 kDa (isoform 1; TRPM2-L), 165.3 kDa (isoform 2), and 95.7 kDa (isoform 3; TRPM2-S) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat hippocampus tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRPM2 in 10 µg of rat hippocampus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN769 04055977311853