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MABF3063-25UL Anti-Tax-1 Antibody, clone Lt-4

Anti-Tax-1, clone Lt-4, Cat. No. MABF3063, is a mouse monoclonal antibody that detects Protein Tax-1 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

MABF3063-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3063-25UL
      DescriptionAnti-Tax-1 Antibody, clone Lt-4
      Alternate Names
      • Protein Tax-1
      • Protein X-LOR
      • Protein PX
      • Trans-activating transcriptional regulatory protein of HTLV-1
      Background InformationProtein Tax-1 (UniProt: P03409; also known as Protein X-LOR, Protein PX, Trans-activating transcriptional regulatory protein of HTLV-1) is encoded by the Tax gene in human. Tax-1 is a homodimeric protein that serves as a transcriptional activator that governs the viral transcription from the 5'LTR via the recruitment of dimers of host phosphorylated CREB1. Together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription. It is reported to shuttle from the host nucleus to the cytoplasm and is found predominantly in the nucleus, where it is equally distributed between the nucleoplasm and the nuclear matrix. Tax-1 is reported to modulate the expression of cellular genes leading to the deregulation of T-cell proliferation, disturbing the integrity of cell cycle checkpoints, the DNA damage response, and apoptotic pathways. It is also shown to act as a ubiquitin E3 ligase and stimulates host IKK complex by catalyzing the assembly of free mixed-linkage polyubiquitin chains, resulting in constitutive activation of NF-kB. Phosphorylation of Tax-1 on threonine 48 is shown to result in the loss of NF-kB activation function. Clone Lt-4 recognizes the Tax-1 protein expressed in HTLV-I-infected cells but does not react with uninfected cell lines and fresh and PHA-activated peripheral blood lymphocytes. (Ref.: Peres, C., et al. (2019). J. Virol. Methods. 274; 113728; Wang C., et al. (2016). PLoS Pathog. 12(4); e1005584; Tie, F., et al (1996). J. Virol. 70(12); 8368-8374; Tanaka, Y., et al. (1991). Int J Cancer. 48(4); 623-630; Lee, B., et al. (1989). Tohoku J. Exp. Med. 157(1); 1-11).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibod yIgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Tax-1, clone Lt-4, Cat. No. MABF3063, is a mouse monoclonal antibody that detects Protein Tax-1 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      • ELISA
      • Immunoprecipitation
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected Protein Tax-1 in Flow Cytometry applications (Peres, C., et al. (2019). J Virol Methods. 274:113728).

      ELISA: A representative lot detected Protein Tax-1 in ELISA applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Protein Tax-1 in Immunoprecipitation applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

      Western Blotting Analysis: A representative lot detected Protein Tax-1in Western Blotting applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11; Tanaka, Y., et al. (1991). Int J Cancer. 48(4):623-30).

      Immunocytochemistry Analysis: A representative lot detected Protein Tax-1 in Immunocytochemistry applications (Lee, B., et al. (1989). Tohoku J Exp Med. 157(1):1-11).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenPartially purified Tax-1 protein preparation from HTLV-1 infected TCL-Kan cells
      EpitopeInternal
      CloneLt-4
      Concentration0.5 mg/ml. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone Lt-4 is a mouse monoclonal antibody that detects Protein Tax-1.
      IsotypeIgG3κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tax
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight60 kDa observed for recombinant protein; 39.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant Human T-cell leukemia virus 1 Protein Tax-1.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant Human T-cell leukemia virus 1 Protein Tax-1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3063-25UL 04065270321749

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