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MABT1514-25UG Anti-Tenascin-R Antibody, clone 23-14

Anti-Tenascin-R, clone 23-14, Cat. No. MABT1514, is a mouse monoclonal antibody that detects Tenascin-R and is tested for use in Immunohistochemistry (Paraffin), Inhibition Assay, and Western Blotting.

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MABT1514-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1514-25UG
      DescriptionAnti-Tenascin-R Antibody, clone 23-14
      Alternate Names
      • TN-R
      • Restrictin
      Background InformationTenascin-R (UniProt: Q00546; also known as TN-R, Restrictin) is encoded by the TNR gene (Gene ID: 396213) in chicken. Tenascins are a family of four large glycoproteins (Tenascin-C, R, X, and W) that share a cysteine-rich N-terminus, a sequence of fibronectin type III domains, and homologies to fibronectin-b at the C-terminus. Tenascin-R (TN-R), the smallest member of the tenascin family that can exist as homodimer or homotrimer. It interacts with different cells and matrix components and is involved in neural cell adhesion and neurite formation. Its interaction with contactin-1 enhances neurite outgrowth. It is expressed weakly in E6 embryo and reaches a maximum at E16 and declines in the adult. Its expression is observed in oligodendrocytes during myelination, and it accumulates at Ranvier nodes and by a subpopulation of neurons, including cerebellar stellate/basket cells, motoneurons in the spinal cord, and hippocampal interneurons. TN-R is synthesized with a signal peptide (aa 1-33), which is subsequently cleaved off to generate the mature form. Its N-terminal region contains a cysteine-rich segment that it shares with other members of tenascin family. Within this segment there is a stretch of 20 amino acids that form three heptad repeats of hydrophobic amino acids in an a-helix that might generate a triple-stranded coiled coil to link three or two TN-R polypeptides to trimers or dimers. It also contains four EGF-like domains and nine fibronectin type-III (Fn-III) domains. Fn-III 9 mediates cell attachment and Fn-III 2-5 are reported to mediate neurofascin binding. It also contains a fibrinogen C-terminal domain (aa 1124-1339) that mediates its interaction with chondroitin Sulfate Proteoglycan 5. (Ref.: Nörenberg, U., et al. (1995). J. Cell Biol. 130(2); 473-484; Rathjen, FG., et al. (1991). Development.113(1); 151-164).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Tenascin-R, clone 23-14, Cat. No. MABT1514, is a mouse monoclonal antibody that detects Tenascin-R and is tested for use in Immunohistochemistry (Paraffin), Inhibition Assay, and Western Blotting.
      Key Applications
      • Western Blotting
      • Inhibition
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Inhibition Assay: A representative lot of this antibody inhibited neural cell and retinal cell attachment to Tenascin-R. (Rathjen, F.G., et al. (1991). Development. 113(1):151-64; Norenberg, U., et al. (1995). J Cell Biol. 130(2):473-84; Norenberg, U., et al. (1996). Int J Dev Neurosci. 14(3):217-31).

      Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Tenascin-R in mouse brain and rat brain tissue sections.

      Western Blotting Analysis: A representative lot detected Tenascin-R in Western Blotting applications (Wintergerst, E.S., et al. (2001). J Neurocytol. 30(4):293-301).

      Immunohistochemistry Analysis: A representative lot detected Tenascin-R in Immunohistochemistry applications (Haunso, A., et al. (2000). Brain Res.;864(1):142-5; Wintergerst, E.S., et al. (2001). J Neurocytol.;30(4):293-301; Geissler, M., et al. (2013). J Neurosci.;33(18):7742-55).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenImmunoaffinity isolate of the monoclonal antibody F11 from detergent extracts of adult chicken brain plasma membrane preparations.
      EpitopeC-terminal half
      Clone23-14
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 23-14 is a mouse monoclonal antibody that detects Tenascin-R. It targets an epitope within the Fibronectin type-III 8 domain from the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Chicken
      • Mouse
      • Human
      Species Reactivity NoteHuman, Chicken, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TNR
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~170 kDa observed; 148.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysates.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected Tenascin-R in Rat brain tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1514-25UG 04065267780641