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MABF62 Anti-Tim3 Antibody, clone 2E2

Anti-Tim3 Antibody, clone 2E2 detects level of Tim3 & has been published & validated for use in WB, FC.

Anti-Tim3 Antibody, clone 2E2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: HAVCR2, TIM3, TIMD3 UniProt Number: Q8TDQ0
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MABF62
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF62
      DescriptionAnti-Tim3 Antibody, clone 2E2
      Alternate Names
      • Hepatitis A virus cellular receptor 2 homolog
      • T-cell immunoglobulin and mucin domain-containing protein 3
      • T-cell membrane protein 3
      • TIMD-3
      • TIM-3
      Background InformationAs a member of the T cell immunoglobulin and mucin domain (TIM) family, Tim-3 is reported to promote or terminate Th1 immunity and is thought to influence a range of inflammatory conditions. It is constitutively expressed in a subset of murine IFNgamma-secreting Th1 cells, but not Th2 cells, in both human and mice. Additionally, Tim-3 is expressed dendritic cells, and by a subset of CD4+ cells where its levels of expression increase upon anti-CD3/28 stimulation. This molecule is also highly expressed in in vitro polarized Th1 cells, and at lower levels in Th17 cells. It is known to synergize with Toll-like receptors and is thought to regulate Th1 and Th17 cytokine secretion.
      References
      Product Information
      FormatPurified
      Control
      • Human thymus tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tim3 Antibody, clone 2E2 detects level of Tim3 & has been published & validated for use in WB, FC.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A 1:1,000 dilution of this antibody detected Tim3 in human monocyte derived dendritic cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to human Tim3.
      Clone2E2
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.
      Gene Symbol
      • HAVCR2
      • TIM3
      • TIMD3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      Molecular Weight~30 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human thymus tissue lysate.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected Tim3 in human thymus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF62 04053252591327

      Documentation

      Anti-Tim3 Antibody, clone 2E2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tim3 Antibody, clone 2E2 Certificates of Analysis

      TitleLot Number
      Anti-Tim3, clone 2E2 - 2906340 2906340
      Anti-Tim3, clone 2E2 - 3532430 3532430
      Anti-Tim3, clone 2E2 - 4191167 4191167
      Anti-Tim3, clone 2E2 -2613622 2613622
      Anti-Tim3, clone 2E2 -2716142 2716142
      Anti-Tim3, clone 2E2 -2733278 2733278
      Anti-Tim3, clone 2E2 Monoclonal Antibody 3064846
      Anti-Tim3, clone 2E2 Monoclonal Antibody 2950550
      Anti-Tim3, clone 2E2 Monoclonal Antibody_2808210 2808210

      References

      Reference overviewPub Med ID
      Validation and comparison of tumor-associated trypsin inhibitor (TATI) immunoassays.
      Elisabete Janeiro,Joana Guimarães,Ulf-Håkan Stenman,Manuela Catarino,Outi Itkonen,Joana Guimar,Ulf-H Stenman
      Clinica chimica acta; international journal of clinical chemistry  413  2012

      Show Abstract       		22507084 22507084