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MABF3203-100UG Anti-Tissue Factor/CD142 Antibody, clone HTF-1

This mouse monoclonal Anti-Tissue Factor/CD142, clone HTF-1, Cat. No. MABF3203 is tested for use in Western Blotting, Inhibition assay, Dot Blot and ELISA for the detection of Tissue Factor/CD142.

NewMABF3203-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3203-100UG
      DescriptionAnti-Tissue Factor/CD142 Antibody, clone HTF-1
      Alternate Names
      • HTF1-7B8
      • TF
      • Coagulation factor III
      • Thromboplastin
      Background InformationTissue factor (UniProt: P13726; also known as TF, Coagulation factor III, Thromboplastin, CD142) is encoded by the F3 gene (Gene ID: 2152) in human. Tissue factor (TF) is an integral membrane protein that serves as a high-affinity receptor and cofactor for factor VII/VIIa. It is expressed on perivascular cells and epithelial cells at organ and body surfaces where it forms a hemostatic barrier. It is also reported to provide hemostatic protection to brain, lung, and heart. In normal healthy individuals, levels of TF are very low. Cell-surface levels of TF do not display any directly correlation with its procoagulant activity indicating that TF exists on the cell surface as dormant (encrypted) and active (de-encrypted) form. This allows for a rapid hemostatic response without a need for transcriptional upregulation of the F3 gene. TF normally exists in a partially encrypted state and its de-encryption on circulating monocytes, platelets or endothelial cells by inflammatory mediators can lead to thrombosis. It initiates blood coagulation by forming a complex with circulating factor VII or VIIa and this complex activates factor IX and X by specific, limited proteolysis. TF expression by nonvascular cells plays an essential role in homeostasis; however, TF expression by vascular cells can induce life-threatening intravascular thrombosis in various diseases, such as sepsis, cancer, and atherosclerosis. Inhibition of TF-Factor VIIa activity is shown to reduce coagulation and mortality in animal models of sepsis. Many cancer cells express large amounts of TF, which contributes to tumor angiogenesis and growth. Cancer cells can release TF-positive microparticles into the circulation that can trigger venous thromboembolism. TF on circulating tumor cells can also coat cells with fibrin that traps them within the microvasculature and promote hematogenous metastasis. (Ref.: Carson, S.D., et al. (1987). Blood. 70(2):490-3; Caldwell, JA., et al. (2010). Lab. Invest. 90; 953-962; Kasthuri, RS., et al. (2009). J. Clin. Oncol. 27(29); 4834-4838; Mackman, N. (2004).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-Tissue Factor/CD142, clone HTF-1, Cat. No. MABF3203 is tested for use in Western Blotting, Inhibition assay, Dot Blot and ELISA for the detection of Tissue Factor/CD142.
      Key Applications
      • Western Blotting
      • ELISA
      • Inhibition
      • Dot Blot
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected Tissue Factor/CD142 in ELISA applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).

      Western Blotting Analysis: A representative lot detected Tissue Factor/CD142 in Western Blotting applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3). .

      Dot Blot: A representative lot detected Tissue Factor/CD142 in Dot Blot applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).

      Inhibition Assay: A representative lot detected Tissue Factor/CD142 in Inhibition applications (Carson, S.D., et al. (1987). Blood. 70(2):490-3).

      Immunofluorescence Microscopy: Faulk, W.P., C.A. Labarrere, and S.D. Carson. 1990. Tissue factor: Identification and characterization of cell types in human placentae. Blood 76:86-96.
      Carson, S.D., and S.J. Pirruccello. 1993. Immunofluorescent studies of tissue factor on U87MG cells: Evidence for non-uniform distribution. Blood Coagulation and Fibrinolysis 4:911-920.

      Flow Cytometry: Carson, S.D., S.J. Pirruccello, and W.D. Haire. 1990. Tissue factor antigen and activity are not expressed on the surface of intact cells isolated from an acute promyelocytic leukemia patient. Thrombos. Res. 59:159-170.

      Immunohistochemistry: Neale, T.J., P.G. Tipping, S.D. Carson, and S.R. Holdsworth. 1988. Participation of cell-mediated immunity in the deposition of fibrin in glomerulonephritis. Lancet, No. 8606, 2:421-424.
      McComb, R.D., K.A. Miller, and S.D. Carson. 1991. Tissue factor antigen in senile plaques of Alzheimer's disease. Am. J. Path. 139:491-494.

      Immunoaffinity purification of the tissue factor protein: Carson, S.D., S.E. Ross, R. Bach, and A. Guha. 1987. An inhibitory monoclonal antibody against human tissue factor. Blood 70:490-493.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenFull length native (purified) human Tissue Factor.
      EpitopeUnknown
      CloneHTF-1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone HTF-1 is a mouse monoclonal antibody that specifically detects Tissue Factor/CD142 protein.
      IsotypeIgG1κ
      Species Reactivity
      • Bovine
      • Human
      Species Reactivity NoteHuman, Bovine.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • F3
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~ 42-48 kDa observed; 33.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A-431cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Tissue Factor/CD142 in A-431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3203-100UG 04065271270008