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05-661-I-100UG Anti-β-Tubulin Antibody, clone AA2

Anti-β-Tubulin, clone AA2, Cat. No. 05-661-I, is a mouse monoclonal antibody that detects β-Tubulin and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

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05-661-I-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue Number05-661-I-100UG
      DescriptionAnti-β-Tubulin Antibody, clone AA2
      Alternate Names
      • Tubulin beta-5 chain
      • beta-Tubulin
      Background InformationTubulin β chain (UniProt: Q2KJD0; also known as Tubulin beta-5 chain) is encoded by the TUBB gene (Gene ID: 615087) in human. Tubulin is the major constituent of microtubules (MTs). MTs are cytoskeletal filaments composed ofa/β-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. a/β heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the β-tubulin subunit facing the microtubule plus end conferring a structural polarity. Tubulin binds two moles of GTP, one at an exchangeable site on the β-chain and one at a non-exchangeable site on the a-chain. Tubulin dimers constantly polymerize and depolymerize, and MTs can undergo rapid cycles of assembly and disassembly. The first stage of MT formation, the nucleation phase, is slow. In the presence of Mg2+ and GTP, a and β tubulins join in an end-to-end manner to form protofilaments with alternating a and β subunits. GTP bound to β-tubulin is hydrolyzed to GDP during or immediately after polymerization. This weakens the binding affinity of tubulin for adjacent molecules and favors depolymerization that contributes to the dynamic behavior of MTs. MTs also undergo treadmilling, in which tubulin molecules bound to GDP are continually lost from the minus end and are replaced by the addition of GTP-bound tubulin molecules to the plus end of the same MT. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins and motor proteins. β-Tubulin is reported to undergo phosphorylation on serine 172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-β-Tubulin, clone AA2, Cat. No. 05-661-I, is a mouse monoclonal antibody that detects β-Tubulin and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected β-Tubulin in Immunofluorescence applications (Abdelfattah, N., et al. (2018). Nat Commun. 9(1):4541; Kelly, J.J., et al. (2015). J Cell Sci. 128(21):3947-60; Moreira, B.P., et al. (2017). J Cell Sci. 130(6):1179-1193; Verbist, K.C., et al. (2016). Nature. 532(7599):389-93).

      Immunocytochemistry Analysis: A representative lot detected β-Tubulin in Immunocytochemistry applications (Verbist, K.C., et al. (2016). Nature. 532(7599):389-93).

      Western Blotting Analysis: A representative lot detected β-Tubulin in Western Blotting applications (Abdelfattah, N., et al. (2018). Nat Commun.;9(1):4541; Cho, J-H., et al. (2017). Nat Commun. 8(1):1848; Moreira, B.P., et al. (2017). J Cell Sci. 130(6):1179-1193; Ohshiro, K., et al. (2020). Genes Cancer. 11(1-2):43-52; Rawlinson, S.M., et al. (2018). Nat Commun. 9(1):3057; Shrestha, P., et al. (2020). Nat Neurosci. 23(2):281-292; Terzaghi, L., et al. (2018). Reproduction. 155(3):273-282; Villar-Fincheira, P., et al. (2021). Front Physiol.12:722528).

      Immunohistochemistry Applications: A representative lot detected β-Tubulin in Immunohistochemistry applications (Cho, J-H., et al. (2017). Nat Commun. 8(1):1848).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified β-tubulin from bovine brain.
      EpitopeC-terminal
      CloneAA2
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone AA2 is a mouse monoclonal antibody that detects β-Tubulin. It targets an epitope within 19 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Bovine
      • Rabbit
      • Rat
      • Mouse
      • Syrian Hamster
      • Human
      Species Reactivity NoteHuman, Bovine, Hamster, Mouse, Rat, Rabbit.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 49.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysates.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected β-Tubulin in A431 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-661-I-100UG 04065267061634

      Documentation

      Anti-β-Tubulin Antibody, clone AA2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-β-Tubulin Antibody, clone AA2 Certificates of Analysis

      TitleLot Number
      Anti-β-Tubulin, clone AA2 - 3956067 3956067
      Anti-β-Tubulin, clone AA2 - 3981709 3981709
      Anti-β-Tubulin, clone AA2 - 4010119 4010119
      Anti-β-Tubulin, clone AA2 - 4010718 4010718
      Anti-β-Tubulin, clone AA2 - 4014162 4014162
      Anti-β-Tubulin, clone AA2 - 4023767 4023767
      Anti-β-Tubulin, clone AA2 - 4036268 4036268
      Anti-β-Tubulin, clone AA2 - 4046621 4046621
      Anti-β-Tubulin, clone AA2 - 4048037 4048037
      Anti-β-Tubulin, clone AA2 - 4049876 4049876
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