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MAB1864 Anti-Tubulin Antibody, clone YL1/2

MAB1864
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, IHC, IP, RIA, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1864
      Replaces04-1117
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tubulin Antibody, clone YL1/2
      Background InformationTubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtubule formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.
      References
      Product Information
      FormatPurified
      Control
      • All tissue
      PresentationPurified rat monoclonal IgG2a in buffer containing phosphate buffered saline (pH 7.4) with 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Tubulin using this Anti-Tubulin Antibody, clone YL1/2 validated for use in ELISA, IH, IP, RIA & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunoprecipitation
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:50-1:100 dilutoin from a previous lot was used on frozen sections.

      ELISA:
      1:100-1:1,000 dilution from a previousl lot was used.

      Immunoprecipitation:
      A previous lot of this antibody was used on Immunoprecipitation.

      Radioimmunoassy:
      A previous lot of this antibody was used on Radioimmuoassy.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenYeast tubulin
      EpitopeThe epitope recognizes a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).
      CloneYL1/2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityMAB1864 recognizes the α subunit of tubulin-specificity binding to so called Tyr-Tubulin, which is produced by post-translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C-terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SUBUNIT STRUCTURE: Dimer of alpha and beta chains.
      TISSUE SPECIFICTY: Expressed at a high level in fetal brain. Ref.7
      PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively By similarity.
      DISEASE: Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM:611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem. Ref.7
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected TUBULIN on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Tubulin Antibody, clone YL1/2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tubulin Antibody, clone YL1/2 Certificates of Analysis

      TitleLot Number
      Anti-Tubulin, clone YL1/2 3062805
      Anti-Tubulin, clone YL1/2 - 2148228 2148228
      Anti-Tubulin, clone YL1/2 - 2358849 2358849
      Anti-Tubulin, clone YL1/2 - 2392242 2392242
      Anti-Tubulin, clone YL1/2 - 2462769 2462769
      Anti-Tubulin, clone YL1/2 - 2041539 2041539
      Anti-Tubulin, clone YL1/2 - 2070803 2070803
      Anti-Tubulin, clone YL1/2 - 2281627 2281627
      Anti-Tubulin, clone YL1/2 - 2508797 2508797
      Anti-Tubulin, clone YL1/2 - 3173830 3173830

      References

      Reference overviewApplicationSpeciesPub Med ID
      Quantitative analysis reveals how EGFR activation and downregulation are coupled in normal but not in cancer cells.
      Capuani, F; Conte, A; Argenzio, E; Marchetti, L; Priami, C; Polo, S; Di Fiore, PP; Sigismund, S; Ciliberto, A
      Nature communications  6  7999  2015

      Show Abstract
      26264748 26264748
      Flattop regulates basal body docking and positioning in mono- and multiciliated cells.
      Gegg, M; Böttcher, A; Burtscher, I; Hasenoeder, S; Van Campenhout, C; Aichler, M; Walch, A; Grant, SG; Lickert, H
      eLife  3  2014

      Show Abstract
      25296022 25296022
      CDK phosphorylation of SLD-2 is required for replication initiation and germline development in C. elegans.
      Gaggioli, V; Zeiser, E; Rivers, D; Bradshaw, CR; Ahringer, J; Zegerman, P
      The Journal of cell biology  204  507-22  2014

      Show Abstract
      24535824 24535824
      The auto-inhibitory domain and ATP-independent microtubule-binding region of Kinesin heavy chain are major functional domains for transport in the Drosophila germline.
      Williams, LS; Ganguly, S; Loiseau, P; Ng, BF; Palacios, IM
      Development (Cambridge, England)  141  176-86  2014

      Show Abstract
      24257625 24257625
      Keratins as the main component for the mechanical integrity of keratinocytes.
      Ramms, L; Fabris, G; Windoffer, R; Schwarz, N; Springer, R; Zhou, C; Lazar, J; Stiefel, S; Hersch, N; Schnakenberg, U; Magin, TM; Leube, RE; Merkel, R; Hoffmann, B
      Proceedings of the National Academy of Sciences of the United States of America  110  18513-8  2013

      Show Abstract
      24167246 24167246
      Interphase cytofission maintains genomic integrity of human cells after failed cytokinesis.
      Choudhary, A; Lera, RF; Martowicz, ML; Oxendine, K; Laffin, JJ; Weaver, BA; Burkard, ME
      Proceedings of the National Academy of Sciences of the United States of America  110  13026-31  2013

      Show Abstract
      Immunofluorescence23878225 23878225
      WAVE/SCAR promotes endocytosis and early endosome morphology in polarized C. elegans epithelia.
      Patel, FB; Soto, MC
      Developmental biology  377  319-32  2013

      Show Abstract
      23510716 23510716
      Regional heterogeneity in murine lung fibroblasts from normal mice or mice exposed once to cigarette smoke.
      Preobrazhenska, O; Wright, JL; Churg, A
      PloS one  7  e39761  2012

      Show Abstract
      Western Blotting22761892 22761892
      A phase 1 dose-escalation study of ARRY-520, a kinesin spindle protein inhibitor, in patients with advanced myeloid leukemias.
      Hanna Jean Khoury,Guillermo Garcia-Manero,Gautam Borthakur,Tapan Kadia,Maria Cielo Foudray,Martha Arellano,Amelia Langston,Beverly Bethelmie-Bryan,Selena Rush,Kevin Litwiler,Sharon Karan,Heidi Simmons,Adam I Marcus,Mieke Ptaszynski,Hagop Kantarjian
      Cancer  118  2012

      Show Abstract
      22139909 22139909
      Stromal interaction molecules 1 and 2 are key regulators of autoreactive T cell activation in murine autoimmune central nervous system inflammation.
      Michael K Schuhmann,David Stegner,Alejandro Berna-Erro,Stefan Bittner,Attila Braun,Christoph Kleinschnitz,Guido Stoll,Heinz Wiendl,Sven G Meuth,Bernhard Nieswandt
      Journal of immunology (Baltimore, Md. : 1950)  184  2010

      Show Abstract
      20028655 20028655

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies