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MABS1683 Anti-UBE3A Antibody, clone 10H7.1

Anti-UBE3A, clone 10H7.1, Cat. No. MABS1683, is a mouse monoclonal antibody that detects Ubiquitin-protein ligase E3A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-UBE3A Antibody, clone 10H7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: UBE3A, E6AP, EPVE6AP, HPVE6A UniProt Number: Q05086
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MABS1683
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1683
      DescriptionAnti-UBE3A Antibody, clone 10H7.1
      Alternate Names
      • Ubiquitin-protein ligase E3A
      • EC: 2.3.2.26
      • E6AP ubiquitin-protein ligase
      • HECT-type ubiquitin transferase E3A
      • Human papillomavirus E6-associated protein
      • Oncogenic protein-associated protein E6-AP
      • Renal carcinoma antigen NY-REN-54
      Background InformationUbiquitin-protein ligase E3A (UniProt: Q05086; also known as EC: 2.3.2.26, E6AP ubiquitin-protein ligase, HECT-type ubiquitin transferase E3A, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54) is encoded by the UBE3A (also known as E6AP, EPVE6AP, HPVE6A) gene (Gene ID: 7337)in human. UBE3A is the E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified, including the RAD23A and RAD23B, MCM7, annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. UBE3A catalyzes the high-risk human papilloma virus (HPV) E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Phosphorylation at Tyr659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells. UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins and it also promotes its own degradation in vivo. It is reported to plays an important role in the regulation of the circadian clock by ubiquitinating the core clock component ARNTL/BMAL1 and leading to its proteasomal degradation. Three isoforms of UBE3A have been reported that are generated via alternative splicing. Mutations in UBE3A gene cause Angelman syndrome that is characterized by severe motor and intellectual retardation, ataxia, hypotonia, and frequent jerky limb movements.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-UBE3A, clone 10H7.1, Cat. No. MABS1683, is a mouse monoclonal antibody that detects Ubiquitin-protein ligase E3A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected UBE3A in 10 µg of HeLa cell lysate.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected UBE3A in human cerebral cortex and human testis tissue.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 101 amino acids from the internal region of human Ubiquitin-protein ligase E3A.
      Clone10H7.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10H7.1 detects Ubiquitin-protein ligase E3A in human cells. it targets an epitope with in 101 amino acids from the internal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UBE3A
      • E6AP
      • EPVE6AP
      • HPVE6A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~100 kDa observed; 100.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC-12 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected UBE3A in 10 µg of PC-12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1683 04054839315992