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ABS1677 Anti-V-ATPase subunit d 2

Detect using this rabbit polyclonal Anti-V-ATPase subunit d 2 Antibody, Cat. No. ABS1677, validated for use in Western Blotting.

Anti-V-ATPase subunit d 2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Atp6v0d2 UniProt Number: Q80SY3
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ABS1677
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1677
      DescriptionAnti-V-ATPase subunit d 2
      Alternate Names
      • V-type proton ATPase subunit d 2
      • Osteoclast-specific vacuolar ATP synthase
      • V-ATPase subunit d 2
      • Vacuolar proton pump subunit d 2
      Background InformationV-type proton ATPase subunit d 2 (UniProt Q80SY3; also known as Osteoclast-specific vacuolar ATP synthase, V-ATPase subunit d 2, Vacuolar proton pump subunit d 2) is encoded by the Atp6v0d2 gene (Gene ID 242341) in murine species. V-ATPase is a proton pump consists of an 8-subunit (subunit A through H) peripheral catalytic V1 complex (V1) and a 6-subunit (a, c, c , d, e, and c' in yeast or Ac45 in higher eukaryotes) catalytic integral membrane V0 proton pore complex. V0 subunit d is a 38 kDa protein located at the cytosolic face of the membrane. There exist two mammalian subunit d isoforms, d1 and d2, encoded by two separate genes, ATP6V0D1 and ATP6V0D1. While d1 is expressed ubiquitously, d2 was expressed in specific tissues, such as kidney, lung, and bone. In addtion, d2 expression level is about 5-fold higher than d1 in osteoclasts. Atp6v0d1-knockout is embryonic lethal in mice, while Atp6v0d2 deletion does not affect V-ATPase function, but decreases cell to cell fusion. V-ATPase subunit d 2 transcription is mediated by transcription factor nuclear factor of activated T cells cytoplasmic 1 (NFATc1) that is essential for osteoclastogenesis.
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect using this rabbit polyclonal Anti-V-ATPase subunit d 2 Antibody, Cat. No. ABS1677, validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected higher V-ATPase subunit d 2 expression in osteoclasts differentiated in culture from murine bone marrow monocyte precursors (BMMs) infected by RACK1-expressing retrovirus due to an upregulated NFATc1 expression caused by RACK1-overexpression (Lin, J., et al. (2015). Sci. Signal. 8(379):ra54).

      Western Blotting Analysis: A representative lot detected less V-ATPase subunit d 2 expression in osteoclasts differentiated in culture from GSK3 -S9A Tg mouse bone marrow monocyte precursors (BMMs) due to a downregulated NFATc1 expression caused by osteoclast-specific GSK3 -S9A mutant expression (Jang, H.D., et a1. (2011). J. Biol. Chem. 286(45):39043-39050).

      Western Blotting Analysis: A representative lot detected V-ATPase subunit d 2 in osteoclasts, but not bone marrow monocyte precursors (BMMs), from wild-type mice, nor in osteoclasts from Atp6v0d2-knock mice (Lee, S.H., et al. (2006). Nat. Med. 12(12):1403-1409).
      Biological Information
      ImmunogenLinear peptide corresponding to human/mouse/rat V-ATPase subunit d 2 C-terminal end sequence.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antiserum was raised against V-ATPase subunit d 2 C-terminal end sequence conserved among human, mouse, and rat species. Specificity was confirmed by the lack of cross-reactivity to mouse Atp6v0d1 overexpressed in 293 cells (Lee, S.H., et al. (2006). Nat. Med. 12(12):1403-1409).
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human, Non-Human Primate, Rat based on 100% sequence homology. Broad species reactivity expected based on high sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Atp6v0d2
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~38 kDa observed. 40.43/40.49/40.52 kDa (human/mouse/rat) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse osteoclasts lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected V-ATPase subunit d 2 in mouse osteoclast lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1677 04054839057175