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627724 Anti-VAMP2 (36-56) Rabbit pAb

Anti-VAMP2 (36-56) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Vesicle-associated Membrane Protein-2, Anti-Synaptobrevin-2

627724
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~12 kDa VAMP2 protein.
      Catalogue Number627724
      Brand Family Calbiochem®
      SynonymsAnti-Vesicle-associated Membrane Protein-2, Anti-Synaptobrevin-2
      Application Data
      Detection of mouse VAMP2 by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-VAMP2 (36-56) Rabbit pAb (Cat. No. 627724) (1:1000). Detection: chemiluminescence.
      References
      ReferencesPevsner, J., et al. 1994. Neuron 13, 353.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Positive controlMouse brain tissue
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:2000, colorimetric)
      Application CommentsSpecificity was confirmed by the reactivity of this antibody with VAMP2-GST fusion protein. The detection of this protein was blocked by preincubation of anti VAMP2 antibody with the immunogen peptide. Weak reactivity with a ~40 kDa protein in mouse liver extracts was also detected. No reactivity with the ~12 kDa protein was seen with mouse liver extracts. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide (QAQVDEVVDIMRVNVDKVLER) corresponding to amino acids 36-56 of rat VAMP2, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). For maximum product recovery after thawing, centrifuge the product vial before removing cap.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      627724 0

      Documentation

      Anti-VAMP2 (36-56) Rabbit pAb Certificates of Analysis

      TitleLot Number
      627724

      References

      Reference overview
      Pevsner, J., et al. 1994. Neuron 13, 353.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2009 JSW
      SynonymsAnti-Vesicle-associated Membrane Protein-2, Anti-Synaptobrevin-2
      ApplicationImmunoblotting (1:2000, colorimetric)
      Application Data
      Detection of mouse VAMP2 by immunoblotting. Sample: Whole tissue extract from mouse brain. Primary antibody: Anti-VAMP2 (36-56) Rabbit pAb (Cat. No. 627724) (1:1000). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~12 kDa VAMP2 protein.
      BackgroundVAMP2 (Vesicle-associated membrane protein-2), also known as synaptobrevin-2, is localized to the cytoplasmic surface of the synaptic vesicle. VAMP2 is involved in vesicular transport and neurotransmission. VAMP2 is also the target of clostridial neurotoxins that block neurotransmitter release from presynaptic terminals.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide (QAQVDEVVDIMRVNVDKVLER) corresponding to amino acids 36-56 of rat VAMP2, conjugated to KLH
      IsotypeIgG
      SpeciesXenopus, bovine, mouse, rat
      Positive controlMouse brain tissue
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Preservative≤0.1% sodium azide
      CommentsSpecificity was confirmed by the reactivity of this antibody with VAMP2-GST fusion protein. The detection of this protein was blocked by preincubation of anti VAMP2 antibody with the immunogen peptide. Weak reactivity with a ~40 kDa protein in mouse liver extracts was also detected. No reactivity with the ~12 kDa protein was seen with mouse liver extracts. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). For maximum product recovery after thawing, centrifuge the product vial before removing cap.
      Toxicity Standard Handling
      ReferencesPevsner, J., et al. 1994. Neuron 13, 353.