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ABN1647-25UL Anti-VGluT1

Anti-VGLUT1, Cat. No. ABN1647, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 1 and has been tested in Immunoautoradiography, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-VGluT1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Slc17a7, Bnpi, Vglut1 UniProt Number: Q62634
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ABN1647-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RImmunoautoradiography, IF, IHC, WBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1647-25UL
      DescriptionAnti-VGluT1
      Alternate Names
      • Vesicular glutamate transporter 1
      • Brain-specific Na(+)-dependent inorganic phosphate cotransporter
      • Solute carrier family 17 member 7
      Background InformationVesicular glutamate transporter 1 (UniProt: also known as VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7) is encoded by the Slc17a7 (also known as Bnpi, Vglut1) gene (Gene ID: 116638) in rat. VGLUT1 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neuronal cells. Glutamate transporters mediate glutamate uptake into synaptic vesicles and are driven by a proton electrochemical gradient generated by the vacuolar H+-ATPase. Two isoforms of VGLUT1 have been reported that are produced by alternative splicing. VGLUT1 is expressed in the cerebellum, cerebral cortex and hippocampus. Expression of isoform VGLUT2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGLUT1 and VGLUT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGLUT2 expression predominates. VGLUT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1. The VGLUT1 knockout mice die at the age when a strong upregulation of VGLUT1 through newly emerging VGLUT1 synapses as well as a replacement of the previously predominant VGLUT2 isoform in cerebellar, hippocampal, and cortical neurons occurs. (Ref.: Wojcik, SM et al. (2004). Proc. Natl. Acad. Sci. USA 101(18): 7158-7163).
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide and 50% glycerol.
      Applications
      ApplicationAnti-VGLUT1, Cat. No. ABN1647, is a highly specific rabbit polyclonal antibody that targets Vesicular glutamate transporter 1 and has been tested in Immunoautoradiography, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunoautoradiography
      • Immunofluorescence
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunoautoradiography Analysis: A representative lot detected VGLUT1 in rat and mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).

      Immunohistochemistry Analysis: A representative lot detected VGLUT1 in prefrontal cortex of a representative control subject and AD patient (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).

      Western Blotting Analysis: A representative lot detected VGLUT1 in glutamate transporter 1 in rat and human putamen extracts (Kashani, A., et. al. (2007). Neurobiol Agining 28(4):568-78) and prefrontal cortex of controls, MCI, early stage Alzheimer (Kashani, A., et. al. (2008). Neurobiol Aging. 29(11):1619-30).

      Immunofluorescence Analysis: A representative lot detected VGLUT1 in mouse striatum and hippocampus tissue (Courtesy of Dr.Salah el mestikawy PhD, at DR1 CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQU - SATT LUTECH).

      Immunofluorescence Analysis: A representative lot detected VGLUT1 in rat striatum (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).

      Immunoautoradiography Analysis: A representative lot detected VGLUT1 in coronal sections of frontal rat brain (Herzog, E., et. al. (2004). Neuroscience. 123(4):983-1002) and rat brain sagittal sections (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).

      Western Blotting Analysis: A representative lot detected VGLUT1 in BON cells stably expressing VGLUT1 (Herzog, E., et. al. (2001). J Neurosci. 21(22):RC181).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of rat Vesicular glutamate transporter 1 (VGLUT1).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Vesicular glutamate transporter 1 in Human, Mouse, and Rat brain. It targets an epitope with in 14 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Slc17a7
      • Bnpi
      • Vglut1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~55 kDa observed; 61.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.
      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected VGLUT1 in 2 0 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1647-25UL 04054839490033