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DR1126 Anti-WARS Mouse mAb (3A12)

This Anti-WARS Mouse mAb (3A12) is validated for use in Immunoblotting, Immunocytochemsitry for the detection of WARS.

Anti-WARS Mouse mAb (3A12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tryptophanyl-tRNA Synthetase

DR1126
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~50-52 kDa WARS protein in HeLa cells.
      Catalogue NumberDR1126
      Brand Family Calbiochem®
      SynonymsAnti-Tryptophanyl-tRNA Synthetase
      Application Data
      Detection of human WARS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-WARS Mouse mAb (3A12) (Cat. No. DR1126) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human WARS by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-WARS Mouse mAb (3A12) (Cat. No. DR1126) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1-5 µg/ml) Immunocytochemsitry (10 µg/ml)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 50-150 human WARS, expressed as a GST fusion protein
      Clone3A12
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1126 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Tryptophanyl-tRNA Synthetase
      ApplicationImmunoblotting (1-5 µg/ml) Immunocytochemsitry (10 µg/ml)
      Application Data
      Detection of human WARS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-WARS Mouse mAb (3A12) (Cat. No. DR1126) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human WARS by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-WARS Mouse mAb (3A12) (Cat. No. DR1126) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50-52 kDa WARS protein.
      BackgroundThis gene product catalyzes the aminoacylation of tRNA by their cognate amino acid. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 50-150 human WARS, expressed as a GST fusion protein
      Clone3A12
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells
      Negative control293T cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review