Millipore Sigma Vibrant Logo

AP1049 Anti-WASL/N-WASP Rabbit pAb

Anti-WASL/N-WASP Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
AP1049
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~55 kDa WASL protein in SiHa cells.
      Catalogue NumberAP1049
      Brand Family Calbiochem®
      Application Data
      Detection of human WASL/N-WASP by immunoblotting. Sample: Whole cell lysate (30 µg) from SiHa cells. Primary antibody: Anti-WASL/N-WASP Rabbit pAb (Cat. No. AP1049) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesLeung, D.W., et al. 2006. Methods Enzymol. 406, 281.
      Rohatgi, R., et al. 1999. Cell 97, 221.
      Miki, H., et al. 1998. Nature 391, 93.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlSiHa cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1 µg/ml)
      Application CommentsAntibody should be titrated for optimal results with individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids near the N-terminus of human WASL/N-WASP
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1049 0

      Documentation

      References

      Reference overview
      Leung, D.W., et al. 2006. Methods Enzymol. 406, 281.
      Rohatgi, R., et al. 1999. Cell 97, 221.
      Miki, H., et al. 1998. Nature 391, 93.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Application Data
      Detection of human WASL/N-WASP by immunoblotting. Sample: Whole cell lysate (30 µg) from SiHa cells. Primary antibody: Anti-WASL/N-WASP Rabbit pAb (Cat. No. AP1049) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~55 kDa WASL/N-WASP protein.
      BackgroundThe Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. Recent studies have demonstrated that these proteins associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product (N-WASP) is a homolog of WASP, however, unlike the latter, it is ubiquitously expressed with the highest level in neural tissues. It has been shown to bind Cdc42 directly and induce formation of long actin microspikes.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids near the N-terminus of human WASL/N-WASP
      IsotypeIgG
      Specieshuman
      Positive controlSiHa cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results with individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLeung, D.W., et al. 2006. Methods Enzymol. 406, 281.
      Rohatgi, R., et al. 1999. Cell 97, 221.
      Miki, H., et al. 1998. Nature 391, 93.