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07-2270 Anti-WNK4 Antibody

This WNK4 antibody is validated for use in WB & IHC for the detection of the WNK4 protein.

Anti-WNK4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: WNK4, PRKWNK4 UniProt Number: Q96J92
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07-2270
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2270
      DescriptionAnti-WNK4 Antibody
      Alternate Names
      • Serine/threonine-protein kinase WNK4
      • Protein kinase lysine-deficient 4
      • Protein kinase with no lysine 4
      Background InformationWNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse kidney cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis WNK4 antibody is validated for use in WB & IHC for the detection of the WNK4 protein.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: 1:1,000 dilution from a previous lot detected WNK4 in normal human kidney tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human WNK4 at the C-terminus.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes WNK4 at the C-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react with rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the WNK family of serine-threonine protein kinases. The kinase is part of the tight junction complex in kidney cells, and regulates the balance between NaCl reabsorption and K(+) secretion. The kinase regulates the activities of several types of ion channels, cotransporters, and exchangers involved in electrolyte flux in epithelial cells. Mutations in this gene result in pseudohypoaldosteronism type IIB.
      Gene Symbol
      • WNK4
      • PRKWNK4
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K+ channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K+ secretion to maintain integrated homeostasis (By similarity).

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      COFACTOR: Magnesium (By similarity).

      ENZYME REGULATION: Activation requires autophosphorylation of Ser-335. Phosphorylation of Ser-331 also promotes increased activity (By similarity).

      SUBUNIT STRUCTURE: Interacts with the C-terminal region of KCNJ1 (By similarity).

      SUBCELLULAR LOCATION: Cell junction › tight junction By similarity. Note: Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity).

      TISSUE SPECIFICITY: Expressed in kidney, colon and skin.

      INVOLVEMENT IN DISEASE: Defects in WNK4 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.

      Contains 1 protein kinase domain.

      CAUTION: Cys-203 is present instead of the conserved Lys which is expected to be an active site residue. Lys-186 appears to fulfill the required catalytic function. UniProtKB Q9JIH7

      SEQUENCE CAUTION: The sequence BAC04669.1 differs from that shown. Reason: Erroneous initiation.

      The sequence CAC48387.1 differs from that shown. Reason: Frameshift at position 4.
      Molecular Weight~160 kDa observed. This protein has 3 isoforms produced by alternative splicing. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse kidney cell lysate.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected WNK4 on 10 µg of mouse kidney cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2270 04053252682810

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-WNK4 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-WNK4 Antibody Certificates of Analysis

      TitleLot Number
      Anti-WNK4 - 2017700 2017700
      Anti-WNK4 - 2291737 2291737
      Anti-WNK4 - 2512124 2512124
      Anti-WNK4 - 3218278 3218278
      Anti-WNK4 - 3599025 3599025
      Anti-WNK4 - 3943324 3943324
      Anti-WNK4 - 4098756 4098756
      Anti-WNK4 - NRG1855956 NRG1855956
      Anti-WNK4 -2618678 2618678
      Anti-WNK4 -2703742 2703742
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