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ABC129 Anti-WWP2 Antibody

ABC129
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC129
      DescriptionAnti-WWP2 Antibody
      Alternate Names
      • NEDD4-like E3 ubiquitin-protein ligase WWP2
      • WW domain-containing protein 2
      Background InformationWW2 (atrophin-1-interacting protein 2) is a member of a large family of NEDD4-like E3 ligases that transfer ubiquitin from E2 ubiquitin-conjugating enzymes to targeted protein substrates. In general, ubiquitination affects protein turnover and activity. As a result, ubiquitin-related enzymes play essential roles in many cellular processes. The WW2 E3 ubiquitin-protein ligase contains a C2 domain at the N-terminus and a HECT (E6AP-type E3 ubiquitin-protein ligase) domain at the C-terminus which interact to inhibit its catalytic activity. Previous studies have suggested that WW2 may play a role in cellular transport, transcription, and T-cell activation. It has also been shown that WW2 regulates the PTEN tumor suppressor and plays an essential role in apoptosis and survival of cancer cells. WW2-deficient mice show abnormal development of craniofacial structures.
      References
      Product Information
      FormatAffinity Purified
      Control
      • mESC lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-WWP2 Antibody is validated for use in Western Blotting, IHC for the detection of WWP2.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: A representative lot of this antibody detected WWP2 in CGR8 ES whole cell lysate and in transiently transfected HEK293 cells (Xu, H. M. et al. (2004). J Biol Chem. 279(22):23495-23503.).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse WWP2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Mouse
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Wwp2
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~100 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~57 kDa and ~60 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mESC lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected WWP2 in 10 µg of mESC lysate (For optimal performance, a range of 1 to 2 µg/mL of this antibody is recommended in Western Blot).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-WWP2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-WWP2 Antibody Certificates of Analysis

      TitleLot Number
      Anti-WWP2 - 3468679 3468679
      Anti-WWP2 - Q2061522 Q2061522
      Anti-WWP2 -2610661 2610661
      Anti-WWP2 -2737225 2737225

      References

      Reference overviewPub Med ID
      Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination.
      Xu, Hui Ming, et al.
      J. Biol. Chem., 279: 23495-503 (2004)  2004

      Show Abstract
      15047715 15047715